LOCUS       pHygG3r__4405_          6039 bp    DNA        circular     19-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     New_DNA(1) from 8 (BsaI) to 4223 (BsaI) 
COMMENT     New_DNA(3) from 12 (BsaI) to 1824 (BsaI) (rev-com)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(2864..3523)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5534..5935
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4662..4712
                     /locus_tag="Synthetic Intron 1"
                     /label="Synthetic Intron 1"
                     /ApEinfo_label="Synthetic Intron 1"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(4556..4661,4713..4841,4893..5023,5075..5299,
                     5351..5470)
                     /locus_tag="NeonGreen_Cel"
                     /label="NeonGreen_Cel"
                     /ApEinfo_label="NeonGreen_Cel"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5300..5350
                     /locus_tag="Synthetic Intron 4"
                     /label="Synthetic Intron 4"
                     /ApEinfo_label="Synthetic Intron 4"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     4523..4555
                     /locus_tag="SV40 nls"
                     /label="SV40 nls"
                     /ApEinfo_label="SV40 nls"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5024..5074
                     /locus_tag="Synthetic Intron 3"
                     /label="Synthetic Intron 3"
                     /ApEinfo_label="Synthetic Intron 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4432..4465)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4368..4432
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3984..4290)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     4486..4519
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4319..4352
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4523..4525
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4842..4892
                     /locus_tag="Synthetic Intron 2"
                     /label="Synthetic Intron 2"
                     /ApEinfo_label="Synthetic Intron 2"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 5471..5527
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           1480..1831
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        24..386
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             429..1454
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     403..423
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    410..416
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden=""
     rep_origin      complement(2079..2766)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cccagttgtc ctcgaacaGG TCcATTTTTG CTTTCGTCGT AAATCTACAC ACGCGTCTCT
       61 TCCGTGCGAG AGTCCAAGCC AGCAGCCAAA TTCGTTGACT GAGTATTCAA CGTTTATACG
      121 TTGTCGGCAA CGAGAAATAG GAAAATGCAT CGGGAAATGT TCTTTTTTCG ATTTTTTCCA
      181 AGGTTTTGAC AAATTTTACC ACGAATTTTG CTATGTTTTC AATTAAAAAA TATGTTATTC
      241 AACTGTTTCT ATGAGGAAAA TAAGGCTTTG CATGTAATTT TCTTATTCAG CATAATTTTT
      301 AATTAATTTG AATTTTCTGT CCTAACGTTT ATTTTGTTTT CTTGGTTATG ACTGATCTGA
      361 AATTAATTTT TGAATTTTAA GGTAATATGT CAGGCGGTGC CGCAAGTTTG TACAAAAAAG
      421 CAGGCTCCAT GAAAAAGCCT GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA
      481 AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA
      541 GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT
      601 ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC
      661 TTGACATTGG GGAATTCAGC GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG
      721 TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG
      781 CCATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC
      841 CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC
      901 ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC
      961 TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG
     1021 ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA
     1081 GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGAGGCCGT
     1141 GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG
     1201 GATCGCCGCG GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT
     1261 TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC
     1321 GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA
     1381 CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA
     1441 GGGCAAAGGA ATAGACCCAG CTTTCTTGTA CAAAGTGGGT CCAATTACTC TTCAACATCC
     1501 CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT
     1561 CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT
     1621 TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT
     1681 AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC
     1741 TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT
     1801 GTTACGTTTC AGTTTATGAC CGCAATTTTT Aatcccgctt tccagtcggg aaacctgtcg
     1861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcga
     1921 attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2341 ccaagctggg ctgtgtgcac gaacccccca ttcagcccga ccgctgcgcc ttatccggta
     2401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2521 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3001 gagatccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3841 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3901 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3961 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4021 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4081 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4141 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4201 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4261 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgt cggagctcAT
     4321 AACTTCGTAT AGCATACATT ATACGAAGTT ATgcggccgc tctagaaCTA GTGGTACCGA
     4381 AGAGCatGCa aCGCGTggtt accTGCAGGC TCTTCGCCAC TTAAGAcccg gGAAGTTCCT
     4441 ATACtatttg aaGAATAGGA ACTTCtgcag gaattcgata tcGGTGAAGT TCCTATTCtc
     4501 tagaaaGTAT AGGAACTTCA AAATGGGATC CCCAAAGAAG AAGCGTAAGG TGTCGATGGT
     4561 GTCGAAGGGA GAAGAGGATA ACATGGCTTC ACTCCCAGCT ACACACGAAC TCCACATCTT
     4621 CGGATCGATC AACGGAGTGG ATTTCGATAT GGTCGGACAA Ggtaagttta aacatatata
     4681 tactaactaa ccctgattat ttaaattttc agGAACTGGA AACCCAAACG ATGGATACGA
     4741 GGAACTCAAC CTCAAGTCGA CAAAGGGAGA TCTGCAATTC TCGCCATGGA TTCTCGTGCC
     4801 ACACATCGGA TACGGATTCC ACCAATACCT CCCATACCCA Ggtaagttta aactgagttc
     4861 tactaactaa cgagtaatat ttaaattttc agATGGAATG TCACCATTCC AAGCTGCCAT
     4921 GGTGGATGGA TCGGGATACC AAGTTCACCG AACAATGCAA TTCGAGGATG GAGCCTCGCT
     4981 CACAGTGAAC TACCGATACA CATACGAGGG ATCGCACATC AAGgtaagtt taaacagttc
     5041 ggtactaact aaccatacat atttaaattt tcagGGAGAG GCTCAAGTTA AGGGAACAGG
     5101 ATTCCCAGCT GATGGACCAG TGATGACAAA CTCACTCACA GCTGCTGATT GGTGCCGATC
     5161 GAAAAAGACA TACCCAAATG ATAAGACAAT CATCTCGACA TTCAAGTGGT CGTACACTAC
     5221 TGGAAACGGA AAGCGATACC GATCGACAGC CCGAACAACA TACACATTCG CTAAGCCAAT
     5281 GGCCGCCAAC TACCTCAAGg taagtttaaa catgatttta ctaactaact aatctgattt
     5341 aaattttcag AATCAACCAA TGTACGTGTT CCGAAAGACA GAACTCAAGC ACTCAAAGAC
     5401 AGAGCTGAAC TTCAAAGAGT GGCAAAAGGC CTTCACAGAT GTGATGGGAA TGGATGAACT
     5461 CTACAAGGCC TCCCGTCGTC GTAAGGCCAA CCCAACCAAG CTCTCCGAGA ACGCCAAGAA
     5521 GCTCGCCAAG tagatcaact gctctaattc catctcgttt ctctcttttc tttcaatctc
     5581 tttacccgtt tctcttcccc tacgtccccc aaacctacca ctgcacagta acacatacac
     5641 attcaacgtg tcacaataaa agcccatcaa caccttctct gttgtttttt ttcttcaaat
     5701 gcattttttt cactttgttt aataattttt acgcatcgga tttgtaggat agtggaaatt
     5761 tgtccttcaa tgcttcaatt gattcgacac ttctcaatat ttttgatcaa taaaccattg
     5821 attttctatt tagaagaaga tcactgtaaa taacaccaaa aacatcacag caaaaaaact
     5881 cccactcaat tatattactg tttttattga aactagaata aatttgaaaa tcttcaaagc
     5941 atacacaatt aaaaaaaacg aaaaaagaaa aagtcccgtt gattctcaaa cttaaatttt
     6001 taaacttgtc tgaaccatgt tgtaagtttg taaaagttt  
//
