LOCUS       pHygR3                  5966 bp    DNA        circular     13-APR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     New_DNA(1) from 8 (BsaI) to 4223 (BsaI) 
COMMENT     New_DNA(3) from 12 (BsaI) to 1824 (BsaI) (rev-com)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(2864..3523)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5704..5862
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3984..4290)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4319..4352
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     4319..4352
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1832..1850
                     /note=") PCR_conditions {primer
                     sequence:TGGTCTCaatcccgctttccagtcggg}"
                     /locus_tag="7192"
                     /label="7192"
                     /ApEinfo_label="7192"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           1480..1831
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4476..4509
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        24..386
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4513..4515
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             429..1454
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4422..4455)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(1458..1478)
                     /locus_tag="attB2"
                     /label="attB2"
                     /ApEinfo_label="attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4368..4422
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     403..423
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     sig_peptide     4522..4544
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1465..1471)
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    410..416
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(2079..2766)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5461..5703
                     /locus_tag="myo-2 3' UTR(1)"
                     /label="myo-2 3' UTR(1)"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  4516..4521
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(4546..4698,4750..4962,5014..5184,5236..5391)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4546..4548
                     /locus_tag="ATG Overlap"
                     /label="ATG Overlap"
                     /ApEinfo_label="ATG Overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 5398..5454
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cccagttgtc ctcgaacaGG TCcATTTTTG CTTTCGTCGT AAATCTACAC ACGCGTCTCT
       61 TCCGTGCGAG AGTCCAAGCC AGCAGCCAAA TTCGTTGACT GAGTATTCAA CGTTTATACG
      121 TTGTCGGCAA CGAGAAATAG GAAAATGCAT CGGGAAATGT TCTTTTTTCG ATTTTTTCCA
      181 AGGTTTTGAC AAATTTTACC ACGAATTTTG CTATGTTTTC AATTAAAAAA TATGTTATTC
      241 AACTGTTTCT ATGAGGAAAA TAAGGCTTTG CATGTAATTT TCTTATTCAG CATAATTTTT
      301 AATTAATTTG AATTTTCTGT CCTAACGTTT ATTTTGTTTT CTTGGTTATG ACTGATCTGA
      361 AATTAATTTT TGAATTTTAA GGTAATATGT CAGGCGGTGC CGCAAGTTTG TACAAAAAAG
      421 CAGGCTCCAT GAAAAAGCCT GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA
      481 AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA
      541 GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT
      601 ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC
      661 TTGACATTGG GGAATTCAGC GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG
      721 TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG
      781 CCATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC
      841 CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC
      901 ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC
      961 TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG
     1021 ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA
     1081 GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGAGGCCGT
     1141 GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG
     1201 GATCGCCGCG GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT
     1261 TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC
     1321 GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA
     1381 CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA
     1441 GGGCAAAGGA ATAGACCCAG CTTTCTTGTA CAAAGTGGGT CCAATTACTC TTCAACATCC
     1501 CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT
     1561 CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT
     1621 TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT
     1681 AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC
     1741 TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT
     1801 GTTACGTTTC AGTTTATGAC CGCAATTTTT Aatcccgctt tccagtcggg aaacctgtcg
     1861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcga
     1921 attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2341 ccaagctggg ctgtgtgcac gaacccccca ttcagcccga ccgctgcgcc ttatccggta
     2401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2521 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3001 gagatccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3841 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3901 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3961 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4021 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4081 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4141 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4201 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4261 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgt cggagctcAT
     4321 AACTTCGTAT AGCATACATT ATACGAAGTT ATgcggccgc tctagaaCTA GTGGCGAAGA
     4381 GCCTGCAggt aaccACGCGt tGCatGCTCT TCGGTAcccg gGAAGTTCCT ATACtatttg
     4441 aaGAATAGGA ACTTCtgcag gaattcgata tcGGTGAAGT TCCTATTCtc tagaaaGTAT
     4501 AGGAACTTCA AAATGGGATC CCCAAAGAAG AAGCGTAAGG TGTCgATGGT TTCCAAGGGA
     4561 GAGGCCGTCA TCAAGGAGTT CATGCGTTTC AAGGTCCACA TGGAGGGATC CATGAACGGA
     4621 CACGAGTTCG AGATCGAGGG AGAGGGAGAG GGACGTCCAT ACGAGGGAAC CCAAACCGCC
     4681 AAGCTCAAGG TCACCAAGgt aagtttaaac atatatatac taactaaccc tgattattta
     4741 aattttcagG GAGGACCACT CCCATTCTCC TGGGACATCC TCTCCCCACA ATTCATGTAC
     4801 GGATCCCGTG CCTTCACCAA GCACCCAGCC GACATCCCAG ACTACTACAA GCAATCCTTC
     4861 CCAGAGGGAT TCAAGTGGGA GCGTGTCATG AACTTCGAGG ACGGAGGAGC CGTCACCGTC
     4921 ACCCAAGACA CCTCCCTCGA GGACGGAACC CTCATCTACA AGgtaagttt aaacagttcg
     4981 gtactaacta accatacata tttaaatttt cagGTCAAGC TCCGTGGAAC CAACTTCCCA
     5041 CCAGACGGAC CAGTCATGCA AAAGAAGACC ATGGGATGGG AGGCCTCCAC CGAGCGTCTC
     5101 TACCCAGAGG ACGGAGTCCT CAAGGGAGAC ATCAAGATGG CCCTCCGTCT CAAGGACGGA
     5161 GGACGTTACC TCGCCGACTT CAAGgtaagt ttaaacatga ttttactaac taactaatct
     5221 gatttaaatt ttcagACCAC CTACAAGGCC AAGAAGCCAG TCCAAATGCC AGGAGCCTAC
     5281 AACGTCGACC GTAAGCTCGA CATCACCTCC CACAACGAGG ACTACACCGT CGTCGAGCAA
     5341 TACGAGCGTT CCGAGGGACG TCACTCCACC GGAGGAATGG ACGAGCTCTA CAAGGCCTCC
     5401 CGTCGTCGTA AGGCCAACCC AACCAAGCTC TCCGAGAACG CCAAGAAGCT CGCCAAGtag
     5461 atcaactgct ctaattccat ctcgtttctc tcttttcttt caatctcttt acccgtttct
     5521 cttcccctac gtcccccaaa cctaccactg cacagtaaca catacacatt caacgtgtca
     5581 caataaaagc ccatcaacac cttctctgtt gttttttttc ttcaaatgca tttttttcac
     5641 tttgtttaat aatttttacg catcggattt gtaggatagt ggaaatttgt ccttcaatgc
     5701 ttcaattgat tcgacacttc tcaatatttt tgatcaataa accattgatt ttctatttag
     5761 aagaagatca ctgtaaataa caccaaaaac atcacagcaa aaaaactccc actcaattat
     5821 attactgttt ttattgaaac tagaataaat ttgaaaatct tcaaagcata cacaattaaa
     5881 aaaaacgaaa aaagaaaaag tcccgttgat tctcaaactt aaatttttaa acttgtctga
     5941 accatgttgt aagtttgtaa aagttt   
//
