LOCUS       pHygR1r__4309_          5952 bp    DNA        circular     06-MAY-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     hygVec PCR from 8 (BsaI) to 5085 (BsaI) 
COMMENT     nls Scarlet PCR from 8 (BsaI) to 950 (BsaI) 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1034..1693)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3617..4018
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2632..2665
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2669..2671
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2154..2460)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4145..4496)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5590..5952)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2578..2611)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4522..5547)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2513..2578
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(2702..2854,2906..3118,3170..3340,3392..3547)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     2669..2701
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 3554..3610
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(249..936)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gatcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
       61 gcggggagag gcggtttgcg tattgggcga attccgcttc ctcgctcact gactcgctgc
      121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaacccccca
      541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1141 catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct ccagatttat
     1201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     2041 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2101 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2161 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2221 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2281 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2341 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2401 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2461 gccgctacag ggcgcgtcgt cggagctcca ccgcggtggc ggccgctcta gaACTAGTGG
     2521 TACCGAAGAG CatGCaaCGC GTggttaccT GCAGGCTCTT CGCCACTTAA GAcccggGAA
     2581 GTTCCTATAC tatttgaaGA ATAGGAACTT Ctgcaggaat tcgatatcGG TGAAGTTCCT
     2641 ATTCtctaga aaGTATAGGA ACTTCAAAAT GGGATCCCCA AAGAAGAAGC GTAAGGTGTC
     2701 GATGGTTTCC AAGGGAGAGG CCGTCATCAA GGAGTTCATG CGTTTCAAGG TCCACATGGA
     2761 GGGATCCATG AACGGACACG AGTTCGAGAT CGAGGGAGAG GGAGAGGGAC GTCCATACGA
     2821 GGGAACCCAA ACCGCCAAGC TCAAGGTCAC CAAGgtaagt ttaaacatat atatactaac
     2881 taaccctgat tatttaaatt ttcagGGAGG ACCACTCCCA TTCTCCTGGG ACATCCTCTC
     2941 CCCACAATTC ATGTACGGAT CCCGTGCCTT CACCAAGCAC CCAGCCGACA TCCCAGACTA
     3001 CTACAAGCAA TCCTTCCCAG AGGGATTCAA GTGGGAGCGT GTCATGAACT TCGAGGACGG
     3061 AGGAGCCGTC ACCGTCACCC AAGACACCTC CCTCGAGGAC GGAACCCTCA TCTACAAGgt
     3121 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TCAAGCTCCG
     3181 TGGAACCAAC TTCCCACCAG ACGGACCAGT CATGCAAAAG AAGACCATGG GATGGGAGGC
     3241 CTCCACCGAG CGTCTCTACC CAGAGGACGG AGTCCTCAAG GGAGACATCA AGATGGCCCT
     3301 CCGTCTCAAG GACGGAGGAC GTTACCTCGC CGACTTCAAG gtaagtttaa acatgatttt
     3361 actaactaac taatctgatt taaattttca gACCACCTAC AAGGCCAAGA AGCCAGTCCA
     3421 AATGCCAGGA GCCTACAACG TCGACCGTAA GCTCGACATC ACCTCCCACA ACGAGGACTA
     3481 CACCGTCGTC GAGCAATACG AGCGTTCCGA GGGACGTCAC TCCACCGGAG GAATGGACGA
     3541 GCTCTACAAG GCCTCCCGTC GTCGTAAGGC CAACCCAACC AAGCTCTCCG AGAACGCCAA
     3601 GAAGCTCGCC AAGtagatca actgctctaa ttccatctcg tttctctctt ttctttcaat
     3661 ctctttaccc gtttctcttc ccctacgtcc cccaaaccta ccactgcaca gtaacacata
     3721 cacattcaac gtgtcacaat aaaagcccat caacaccttc tctgttgttt tttttcttca
     3781 aatgcatttt tttcactttg tttaataatt tttacgcatc ggatttgtag gatagtggaa
     3841 atttgtcctt caatgcttca attgattcga cacttctcaa tatttttgat caataaacca
     3901 ttgattttct atttagaaga agatcactgt aaataacacc aaaaacatca cagcaaaaaa
     3961 actcccactc aattatatta ctgtttttat tgaaactaga ataaatttga aaatcttcaa
     4021 agcatacaca attaaaaaaa acgaaaaaag aaaaagtccc gttgattctc aaacttaaat
     4081 ttttaaactt gtctgaacca tgttgtaagt ttgtaaaagt ttcccagttg tcctcgaaca
     4141 GGTCTAAAAA TTGCGGTCAT AAACTGAAAC GTAACATATG ATAAGGTATT TTGTGTGCGG
     4201 TTTTTTCTAT GATGTTTCAA AAAAATTTAT CAGAAGTAAA AAACATAAGA AGTGTACACA
     4261 GAACATTGTG TAGATTTTGG GATAGAATTA TTATTAATGG GGGGAGGGAG CACAATTTTT
     4321 TTCGACTTTT TATTACGAAT TAATTCTATT TTTGAATCTA CACAATTTCA TTGTTAGAGG
     4381 TGACTTAAAA GAAGCTAAAA AACAAAGAAA TTAAGAGAAG TTTTTTGATA ATAACAAAAA
     4441 TAGGGGGTGG GAGCACAGGG AGAAAGAGCA TGTAGGGATG TTGAAGAGTA ATTGGACCCA
     4501 CTTTGTACAA GAAAGCTGGG TCTATTCCTT TGCCCTCGGA CGAGTGCTGG GGCGTCGGTT
     4561 TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC
     4621 GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG
     4681 CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC
     4741 AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT GCCTCCGCTC
     4801 GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC CTCCAGAAGA AGATGTTGGC
     4861 GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG TCAATGACCG CTGTTATGCG
     4921 GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC
     4981 GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC
     5041 GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG CGCATATGAA
     5101 ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG AATGGGCCGA ACCCGCTCGT
     5161 CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC GCGACCGGCT GCAGAACAGC
     5221 GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA
     5281 ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC
     5341 CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC AGCTATTTAC
     5401 CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA GCACGAGATT CTTCGCCCTC
     5461 CGAGAGCTGC ATCAGGTCGG AGACGCTGTC GAACTTTTCG ATCAGAAACT TCTCGACAGA
     5521 CGTCGCGGTG AGTTCAGGCT TTTTCATGGA GCCTGCTTTT TTGTACAAAC TTGCGGCACC
     5581 GCCTGACATA TTACCTTAAA ATTCAAAAAT TAATTTCAGA TCAGTCATAA CCAAGAAAAC
     5641 AAAATAAACG TTAGGACAGA AAATTCAAAT TAATTAAAAA TTATGCTGAA TAAGAAAATT
     5701 ACATGCAAAG CCTTATTTTC CTCATAGAAA CAGTTGAATA ACATATTTTT TAATTGAAAA
     5761 CATAGCAAAA TTCGTGGTAA AATTTGTCAA AACCTTGGAA AAAATCGAAA AAAGAACATT
     5821 TCCCGATGCA TTTTCCTATT TCTCGTTGCC GACAACGTAT AAACGTTGAA TACTCAGTCA
     5881 ACGAATTTGG CTGCTGGCTT GGACTCTCGC ACGGAAGAGA CGCGTGTGTA GATTTACGAC
     5941 GAAAGCAAAA AT    
//
