LOCUS       DR274_TGG_ATG_me        2548 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1556..2371)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2542..2548
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..417
                     /locus_tag="mec-4p"
                     /label="mec-4p"
                     /ApEinfo_label="mec-4p"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(906..1493)
                     /locus_tag="origin"
                     /label="origin"
                     /ApEinfo_label="origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2450..2469
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(849..866)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    668..674
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    445..658
                     /locus_tag="mec-4p(1)"
                     /label="mec-4p(1)"
                     /ApEinfo_label="mec-4p"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    418..444
                     /locus_tag="Artifical mec-3 unc-86 binding sequence"
                     /label="Artifical mec-3 unc-86 binding sequence"
                     /ApEinfo_label="Artifical mec-3 unc-86 binding sequence"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gTGGggttcc ggagcagttc aagacaactt ttgtcaatgg caagctagtc actgtcgtct
       61 cggacatttg ctaaatcttc tcacgtcata accttatttt tcaacttcat gaactatccc
      121 ctcgaacttt tttatttgtc cctgtctaca attctttata agcaattgag acacactttg
      181 ctttattaaa taataccgat tttgttttgt atatgcattg actggtgctc aactattttt
      241 attgaaaaaa ttacttttta ctgtctcgtc gaataaaagt gatcccttcc aaaaaatagt
      301 tattcatgtt tgaaggtttg tgatcgtaga acaattgcac cgaatgtagt gtaaaaaaaa
      361 ttccagaatg ttcaaataca tttccaccct caccagaaca tctcaaacac caggcactgg
      421 tattctatac atttcttatg atccatttca acacactttc atggatctta tcttgcctca
      481 aaaccatcag ctttcttctc attctacaag tatgaaacaa tctactattc tatacattta
      541 taatgtaatg gaaccatgtg aagacatgag caagatcatt gtcgtcagcg actacccctc
      601 cttcattttt cttagagtat cagtttgcta atgctatttt ttttatcgct atcaagtttc
      661 agaATGaGAA GAGCAAGCTT GGATCGACGA GAGCAGCGCG ACTGGATCTG TCGCCCGTCT
      721 CAAACGCAAC CCTCCGGCGG TCGCATATCA TTCAGGACGA GCCTCAGACT CCAGCGTAAC
      781 TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG ATCAGCTAGA ATCAAGCTGA
      841 CTAGATAAAC TGGCCGTCGT TTTACACGGG TGGGCCTTTC TTCGGTAGAA AATCAAAGGA
      901 TCTTCttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaggtaactg
     1021 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1441 gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaAACGCCA
     1501 GCAACGCAGA AAGGCCCACC CGAAGGTGAG CCAGGTGATT ACATTTAGGT CCTCATTAGA
     1561 AAAACTCATC GAGCATCAAG TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT
     1621 ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA
     1681 TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA
     1741 ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT
     1801 CCGGTGAGAA TGGCAAGAGT TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT
     1861 TACGCTCGTC ATCAAAATCA CTCGCACCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT
     1921 GAGCGAGACG AAATACGCGA TCGCCGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA
     1981 ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT
     2041 CTAATACCTG GAATGCTGTT TTCCCTGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG
     2101 GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGCC
     2161 TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT
     2221 CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT
     2281 CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCTTCA
     2341 AGCAAGACGT TTCCCGTTGA ATATGGCTCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
     2401 ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAg ttggaacctc
     2461 ttacgtgccG ATCAAGTCAA AAGCCTCCGG TCGGAGGCTT TTGACTTTCT GCTATGGAGG
     2521 TCAGGTATGA TTTttGAATT CGCTCTTC   
//
