LOCUS       DR274_TGG_ATG_F4        3889 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        104..2106
                     /locus_tag="F49H12.4p"
                     /label="F49H12.4p"
                     /ApEinfo_label="F49H12.4p"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2996..3811)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2346..2675)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2676..2933)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2289..2306)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2108..2114
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCG TGGtttgaaa aatgattacg
      121 ataacctgag ttctttcgtt gaaaaaaaat ggcctcgatt attttcattt ttacgagcac
      181 gacttaccca ttaaaattaa aatgagcatg tttgatttgt cttatcccaa tgaaagcgga
      241 aaacctaatc ctccaacaag cttgacatat tattcttggg taaacaaaaa gctgaaacag
      301 ttcctctatt tttttttgtt tttcatcttt tctttgaaac ccgttttgat tattcatcgg
      361 atggagattg gtttcttaaa aaaatttcca ttaatgagaa aatttccaaa ctgaactctt
      421 gaactttcga tttgaagatc tggaatttta gattttattc taatgttttt tttttcagtt
      481 aagcgaaatt taaatatttt aacatttctg aaaaaatttt acttataaaa tctaatactt
      541 caattttgaa aaaaaaaaat tccaaaaact actgatatat attttcaaat aattttttgt
      601 ttattagctc tttgaaaaaa atataacgaa gtcatttttt ttagatattg aagcgtatta
      661 agttgccaaa aactacatcg attactacac acttttaata tgaaaactta cgcttcttcc
      721 attaataaca cacgtcatgt tcttagtatt gagatataca ttactcactt taatcccaaa
      781 cttctaattt ccgatcccaa tcggagcgtt tcatatgtcg ctccactgac ttccgatttg
      841 aaaagcatgg gcattttgct tcattatacg aagtggaggc gtaattaggt tagaacgtca
      901 gtttgatcca tctccaattt tttcctcatt ttttatgatg acaattgttt ggagtataat
      961 aattcttaaa ggtggagtag cgcgagttga tatgttgtag aaatgacaaa atatcatagt
     1021 aaaaatttaa aagaattgac agtcaaaaaa tgacaatctc tgagtttttg caacttaaaa
     1081 aaaaatcata gcactgtcaa atccatacag tgtttgaata ttaatacttg aaacataagt
     1141 tggccttaaa attggaaaaa gttgtttctt taatcgtttt ccataaaaat gacaaaaacc
     1201 taggtaattg tggtaccata tgcatagttt taagcaattt tcccacatga cctatgaata
     1261 taaaaataat ataaaacgtt agactaagta attccagaca aaaacgtagt gtttctttat
     1321 agtgatcgtt cgattaatca tcacaaatct ccatatgata atcttccgcc gtttttaacc
     1381 attcattggc ttttcttcgg ctcagtttgt catcacctca gatatccagc ctccagctgc
     1441 ccgctaatct tgatttcttc tcttacttgt ttttttttcg acgataattc tgattctaat
     1501 tccacctttt ctcttgtctt ctaattcatt gttattcaat tttaatagtc atggttgctc
     1561 ggtttgtaaa tgtcacaacg cacaggtttc acatgaagct gccagaatca tcagtggcat
     1621 cttccacaac ggatagcaca gccggctttg ttgagatgaa tctgacaaca acagtatggc
     1681 cgagaaaaaa agttggagga gttaatcctt ttcttggtat gttttggaaa atggctaaat
     1741 atcatagtaa aactttttgg aaattgtgta ggaagtggca aaaacctggt aattgacact
     1801 ttttgactgt caataaaaat tatcaaaaaa tgtttgggaa gttattctat gatatttggg
     1861 cattttggca ccatagcaga ggtttcaaca attttcccac tggcggaaac gatttagaaa
     1921 acgttttctg ccagcacttt tggcttgcat aactgatgaa tatgtacctg ttagaattgc
     1981 aataaatcat ttctctaatg ctacctttgc gtggaaaacg gacatttggc ctctatctca
     2041 cccccactat ttgagatctt aggaatattt ctcgccttac ttcattcctc aaagaaaata
     2101 gacATGaGAA GAGCAAGCTT GGATCGACGA GAGCAGCGCG ACTGGATCTG TCGCCCGTCT
     2161 CAAACGCAAC CCTCCGGCGG TCGCATATCA TTCAGGACGA GCCTCAGACT CCAGCGTAAC
     2221 TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG ATCAGCTAGA ATCAAGCTGA
     2281 CTAGATAAAC TGGCCGTCGT TTTACACGGG TGGGCCTTTC TTCGGTAGAA AATCAAAGGA
     2341 TCTTCttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaggtaactg
     2461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2881 gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaAACGCCA
     2941 GCAACGCAGA AAGGCCCACC CGAAGGTGAG CCAGGTGATT ACATTTAGGT CCTCATTAGA
     3001 AAAACTCATC GAGCATCAAG TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT
     3061 ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA
     3121 TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA
     3181 ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT
     3241 CCGGTGAGAA TGGCAAGAGT TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT
     3301 TACGCTCGTC ATCAAAATCA CTCGCACCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT
     3361 GAGCGAGACG AAATACGCGA TCGCCGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA
     3421 ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT
     3481 CTAATACCTG GAATGCTGTT TTCCCTGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG
     3541 GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGCC
     3601 TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT
     3661 CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT
     3721 CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCTTCA
     3781 AGCAAGACGT TTCCCGTTGA ATATGGCTCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
     3841 ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAA 
//
