LOCUS       DR274_TGG_ATG_rp        2705 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1812..2627)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        103..919
                     /locus_tag="rpl-27p"
                     /label="rpl-27p"
                     /ApEinfo_label="rpl-27p"
                     /ApEinfo_fwdcolor="#935100"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1162..1491)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1105..1122)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    924..930
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1492..1749)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCg TGGcccaatt ctgatggttc
      121 ttccATTTCT AAATTTTAGT TTAATAAAAA AATTTTAAAA TAATACGAGT TTTGAGACTA
      181 ACTTTCAATA AAAACCaaAA AAAATTGCTT CAAAAAAGAG tTCGCGCCGA TTTACGATAA
      241 ACACAGCGCG CTACGACACT CCGAAAACCG CGCCGCACCA TTACATTCTC CGCCCCACCG
      301 CACGGTTTGC GGAAGGCGAT GAAATTGCTG GGCCTAAAAT TTTAACCCTT TTTTTCCCGT
      361 TTTTCAACTG TTTTCCCGAT TTTTTCGATA TTTTTTTCAG GTTTTTAGTT AGAAAAGTAT
      421 ATAAAAATTG CATTATTTGT AGTTTTCGCT CGTAATTTGT GATAGAAAAC GGCCAATTTG
      481 CCTAGATTTG TGATTCTACC AAGTGGAATT TTTGGGAAAA TATCAATATT CCACGATTTT
      541 TATTCAAATC ATAACGTTTC GAAATTATCA GCTTAAATAA AACTAAAATA AATAATAAAA
      601 TACTAAAAAC CCTGAATTTC TCGGTTCTCG GTTCAGCTTG AGTTCGTTCT ATACACGTTA
      661 TCGATTTTTG TCATTTCGTT TAATCATGGG TTTTGATTCA TTTTAAATAT GAAATTTGAT
      721 TTATTCCTCG TTTCGAAGTT GGTTTGTCTT GTCCGGCCGG TAAACAAATG TTATTAAAAA
      781 AATAATAAAT TTCGAAATTT TTCGTAATGT TCCGCAATCG TGTTTTTCCG TTTATCTCGT
      841 CTTCCCTTCt TTCAAATATA CTATTTATTT TCTCAATTCA CATATTTTCA GCCAGCCCcG
      901 CTCAACAAGT GGAATAAAAA TGaGAAGAGC AAGCTTGGAT CGACGAGAGC AGCGCGACTG
      961 GATCTGTCGC CCGTCTCAAA CGCAACCCTC CGGCGGTCGC ATATCATTCA GGACGAGCCT
     1021 CAGACTCCAG CGTAACTGGA CTGCAATCAA CTCACTGGCT CACCTTCCGG TCCACGATCA
     1081 GCTAGAATCA AGCTGACTAG ATAAACTGGC CGTCGTTTTA CACGGGTGGG CCTTTCTTCG
     1141 GTAGAAAATC AAAGGATCTT Cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1681 gggtttcgcc acctctgact tgagcatcga tttttgtgat gctcgtcagg ggggcggagc
     1741 ctatggaaaA ACGCCAGCAA CGCAGAAAGG CCCACCCGAA GGTGAGCCAG GTGATTACAT
     1801 TTAGGTCCTC ATTAGAAAAA CTCATCGAGC ATCAAGTGAA ACTGCAATTT ATTCATATCA
     1861 GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG
     1921 AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA
     1981 TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA
     2041 TGAGTGACGA CTGAATCCGG TGAGAATGGC AAGAGTTTAT GCATTTCTTT CCAGACTTGT
     2101 TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CACCAACCAA ACCGTTATTC
     2161 ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC CGTTAAAAGG ACAATTACAA
     2221 ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT
     2281 GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CTGGGATCGC AGTGGTGAGT
     2341 AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC
     2401 GTCAGCCAGT TTAGCCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA
     2461 TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT
     2521 GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA
     2581 TTTAATCGCG GCTTCAAGCA AGACGTTTCC CGTTGAATAT GGCTCATTTT AGCTTCCTTA
     2641 GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG
     2701 TGAAA     
//
