LOCUS       DR274_TGG_ATG_nh        3470 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2577..3392)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2257..2514)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    104..1684
                     /locus_tag="Nhx-2 promoter"
                     /label="Nhx-2 promoter"
                     /ApEinfo_label="Nhx-2 promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1927..2256)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1870..1887)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1689..1695
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCg TGGCCTTCCA AGTTATTGAC
      121 AAGTATTAAC tgcacttctc ataaaaattt ttcaaataga tctatttagt ctggaatagg
      181 aatacttttg gcaattttct tgagaatcat agaagacgaa tttctgaact gctctttgtg
      241 aagccacgcc catatattgg catttttgta tccaattttt tgtagagtac ctgaatcatt
      301 atcactacat ctgctaaaca atatatggaa gttctatgag aggtgaaagg taaatcatat
      361 aacaactgta ataacataga aatgattgta gtatatttcc aattaggctg agttgatctt
      421 attgaagagt ttgaatcata gcaatttttt cctcttttag tatttttcaa aacaaatttt
      481 cacaatgtta gatgtagtat ttagcattaa ggcatctgta atctcccaga aatgttttgg
      541 gaataaaacc ctttgactct ttggtccttt tttgctccaa ataatatctg attttaaccc
      601 gttctgatct actttttacc tttcgatcgt cacatccgcg tacacgtttc tcttgctctc
      661 aattgcttat ctttatggcc ttttccccca ctttttgtcg actatcatgc ggacgtggct
      721 tccttgctcc ctcccggttt ttttgaaaac acttgtgaat ttattttttg tggtcaaaat
      781 tacgaaacat ttgaattctc aatctattta tagaccgggt gtggtatgtt atgccatgtt
      841 tttgtcattg ccaaatgaac atcgatattt taattttctg aacttaccgt accccctgcg
      901 ttactatttt atttttaatt tgtataattt cattgctata atttgttgtc cttgaaacat
      961 tgaagatatc gaagaaataa tgacaaaaat cagatagaac aatttttcct actcatcaac
     1021 tcacattttt ctattttctc ttgtgcgaaa aaaatagtat attgggaata tttatagtat
     1081 tgcaactttt ctcttagttc gcagagttgc aatactacta gatccctaat agaggaaata
     1141 tggtgaccag ctttttgcaa ttggaaagtt gggaaaaaag tatatatatt aatagactta
     1201 cactcttgta gttcgtagaa tttttcagga aatctgacac gcaagacttt ttcaaaaagt
     1261 tttttttttt aatttggcaa tctgccaaaa cgtttactga acattttgaa aacatctacc
     1321 agtaccttta acacaacgaa aaattgttct aagcaatatt aactgtgata attgatgtta
     1381 agacctgaaa tttaatcaca aatttcacct ggaaatgaca gtaatcggct tctaaatact
     1441 gtcgacacaa gtgccatacc taagattcca atcctattac catgtttccc aatttcattc
     1501 tctttcgtat ctctatttct cttctttatt atcaattact ttttatcagt tcttcgtttg
     1561 ctttaacatc aagagcacat agcgctctct cgtctctctc tcatgctctt tttgaacttt
     1621 tacaaaaaac tatttccggt tgttttgatt cttggaattt gaaataattt tcagtgatta
     1681 aatcATGaGA AGAGCAAGCT TGGATCGACG AGAGCAGCGC GACTGGATCT GTCGCCCGTC
     1741 TCAAACGCAA CCCTCCGGCG GTCGCATATC ATTCAGGACG AGCCTCAGAC TCCAGCGTAA
     1801 CTGGACTGCA ATCAACTCAC TGGCTCACCT TCCGGTCCAC GATCAGCTAG AATCAAGCTG
     1861 ACTAGATAAA CTGGCCGTCG TTTTACACGG GTGGGCCTTT CTTCGGTAGA AAATCAAAGG
     1921 ATCTTCttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaggtaact
     2041 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaAACGCC
     2521 AGCAACGCAG AAAGGCCCAC CCGAAGGTGA GCCAGGTGAT TACATTTAGG TCCTCATTAG
     2581 AAAAACTCAT CGAGCATCAA GTGAAACTGC AATTTATTCA TATCAGGATT ATCAATACCA
     2641 TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA GTTCCATAGG
     2701 ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT ACAACCTATT
     2761 AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT GACGACTGAA
     2821 TCCGGTGAGA ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC AGGCCAGCCA
     2881 TTACGCTCGT CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG TGATTGCGCC
     2941 TGAGCGAGAC GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG AATCGAATGC
     3001 AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC AGGATATTCT
     3061 TCTAATACCT GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA TGCATCATCA
     3121 GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG CCAGTTTAGC
     3181 CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT CAGAAACAAC
     3241 TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG CCCGACATTA
     3301 TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA TCGCGGCTTC
     3361 AAGCAAGACG TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC
     3421 GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA 
//
