LOCUS       DR274_TGG_ATG_my        2865 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1972..2787)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    596..1079
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    104..595
                     /locus_tag="pMYO-2(1)"
                     /label="pMYO-2(1)"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1322..1651)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1084..1090
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1652..1909)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1265..1282)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCg TGGcatttta tatctgagta
      121 gtatcctttg ctttaaatgt ccataaaact aattttataa tcaataaaac aacgtttgta
      181 aatcaactga gtttacaagt agagacattg agggatactt tcactatgct aaagtgataa
      241 tcgaccaaat aataacttca ctttggtatt tattcctgtc ttataaatgt tatgtatgaa
      301 ttaaattcat atgcatatgg ctcactctga caatttttaa taatcttcca gatcaatatt
      361 gactaccgat gcgggtggtc ttttgctttg aattctgctg aactttacac cccgaacagc
      421 aatgtgtgct tcagcctaaa aaaaagtaag tgtgttaatc agtgcccccg attcttcatt
      481 ttttgcccct ctctcccgtt tcgtcggcaa aagaagagaa aataaagata agtctcaaga
      541 taggttggta atcgctaaag tggttgtgtg gataagagta gcaaaatggc aggaggagca
      601 ctttgcgcgc acacactgta ctcattgttc tggataaaat tctctcgttg tttgccgtcg
      661 gatgtctgcc tctctgcatt gagccggctt cttcactatc tttagttaac ctaaaatgcc
      721 gtttcttttc tcgtatcccc actatcccgt tgaggttctc tgctctcttc gctccctacc
      781 gccagcgagc aactatccgt gggggcgcct tgctcggaag atggggggga agaaagaaga
      841 tttttgctat ttgcacttga gaaagagact tttcctgcgt cgatggttag agaacagtgt
      901 gcagacactt ttcagctacc tagaattaca attggatatc cccgcctccc aatccaccca
      961 cccagggaaa aagaagggct cgccgaaaat caaagttatc tccaggctcg cgcatcccac
     1021 cgagcggttg acttctctcc accacttttc attttaaccc tcgatcgtca gacacagaaA
     1081 TGaGAAGAGC AAGCTTGGAT CGACGAGAGC AGCGCGACTG GATCTGTCGC CCGTCTCAAA
     1141 CGCAACCCTC CGGCGGTCGC ATATCATTCA GGACGAGCCT CAGACTCCAG CGTAACTGGA
     1201 CTGCAATCAA CTCACTGGCT CACCTTCCGG TCCACGATCA GCTAGAATCA AGCTGACTAG
     1261 ATAAACTGGC CGTCGTTTTA CACGGGTGGG CCTTTCTTCG GTAGAAAATC AAAGGATCTT
     1321 Cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgagg taactggctt
     1441 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1861 tgagcatcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaA ACGCCAGCAA
     1921 CGCAGAAAGG CCCACCCGAA GGTGAGCCAG GTGATTACAT TTAGGTCCTC ATTAGAAAAA
     1981 CTCATCGAGC ATCAAGTGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT
     2041 TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC
     2101 AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT
     2161 CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG
     2221 TGAGAATGGC AAGAGTTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG
     2281 CTCGTCATCA AAATCACTCG CACCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC
     2341 GAGACGAAAT ACGCGATCGC CGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG
     2401 GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA
     2461 TACCTGGAAT GCTGTTTTCC CTGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT
     2521 ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGCCTGAC
     2581 CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG
     2641 CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG
     2701 AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCTTCAAGCA
     2761 AGACGTTTCC CGTTGAATAT GGCTCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA
     2821 CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAA 
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