LOCUS       NM4037                  2390 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1497..2312)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        104..604
                     /locus_tag="mex-5 promoter"
                     /label="mex-5 promoter"
                     /ApEinfo_label="mex-5 promoter"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(847..1176)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(790..807)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    609..615
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1177..1434)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCg TGGcacaacg gcaaaATATC
      121 AGTTTTTAAA AAATTAAACC ATAAAACAAA TAATATAACC CAATTTTTAC ATCAAACCAC
      181 AAGAAAAAAA TACATTTGGG CCCACGGATA AAGAAATTAA AAAAATACAT TTTTTAAAGG
      241 CGCACCGAAT TAAAATTCAT TTGGGTCTTA CCGCGTATAC CGTACTCCGT TTGTTTGATC
      301 ATTTTTGTCA GCGCTGGCGG TTGTTTTTTC ATTTCATTTC TGCTTCAAAG ACGTTTTCTC
      361 GAATAATTTT TCGTTTATTC TCTTTTTTAA AATTAATTTC TAGCCGTAAA TGTTATAAAT
      421 TCACCCATTT AACGCAAATT TCATGGTAAT CTCATGGAAA AATGCAGTTT CTTTGTTAAA
      481 GAAAGCTTAA ATAGCAAAAA TTCCCCGACT TTCCCCAAAA TCCTGCTCGA TTTTCCGTTT
      541 TCTCATTGTA TTCTCTCTTA ATTAATTTTA TCGATAATCA ATTGAATGTT TCAGACAGAG
      601 AAAAATGaGA AGAGCAAGCT TGGATCGACG AGAGCAGCGC GACTGGATCT GTCGCCCGTC
      661 TCAAACGCAA CCCTCCGGCG GTCGCATATC ATTCAGGACG AGCCTCAGAC TCCAGCGTAA
      721 CTGGACTGCA ATCAACTCAC TGGCTCACCT TCCGGTCCAC GATCAGCTAG AATCAAGCTG
      781 ACTAGATAAA CTGGCCGTCG TTTTACACGG GTGGGCCTTT CTTCGGTAGA AAATCAAAGG
      841 ATCTTCttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaggtaact
      961 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1381 tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaAACGCC
     1441 AGCAACGCAG AAAGGCCCAC CCGAAGGTGA GCCAGGTGAT TACATTTAGG TCCTCATTAG
     1501 AAAAACTCAT CGAGCATCAA GTGAAACTGC AATTTATTCA TATCAGGATT ATCAATACCA
     1561 TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA GTTCCATAGG
     1621 ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT ACAACCTATT
     1681 AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT GACGACTGAA
     1741 TCCGGTGAGA ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC AGGCCAGCCA
     1801 TTACGCTCGT CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG TGATTGCGCC
     1861 TGAGCGAGAC GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG AATCGAATGC
     1921 AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC AGGATATTCT
     1981 TCTAATACCT GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA TGCATCATCA
     2041 GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG CCAGTTTAGC
     2101 CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT CAGAAACAAC
     2161 TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG CCCGACATTA
     2221 TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA TCGCGGCTTC
     2281 AAGCAAGACG TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC
     2341 GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA 
//
