LOCUS       DR274_ATG_GGT__B        1921 bp    DNA        circular     30-JUL-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Ligation reaction:
COMMENT     DR274 FP-BsaI [3469] from 365 (ApaLI) to 1694 (BamHI)
COMMENT     DR274 TGG GGT -BsaI [3701] from 1695 (BamHI) to 364 (ApaLI)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(321..1136)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1535..1552)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1592..1921)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1354..1360
                     /locus_tag="<SapI"
                     /label="<SapI"
                     /ApEinfo_label="<SapI"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(1..258)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1215..1234
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1307..1313
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1319..1324
                     /locus_tag="<BsaI"
                     /label="<BsaI"
                     /ApEinfo_label="<BsaI"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="#ff9300"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1350..1352
                     /locus_tag="GGT SapI overlap"
                     /label="GGT SapI overlap"
                     /ApEinfo_label="GGT SapI overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1343..1348
                     /locus_tag="BsaI>"
                     /label="BsaI>"
                     /ApEinfo_label="BsaI>"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="#ff9300"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
       61 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      121 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      181 agtcctgtcg ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg
      241 gggcggagcc tatggaaaAA CGCCAGCAAC GCAGAAAGGC CCACCCGAAG GTGAGCCAGG
      301 TGATTACATT TAGGTCCTCA TTAGAAAAAC TCATCGAGCA TCAAGTGAAA CTGCAATTTA
      361 TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA
      421 AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT ATCGGTCTGC GATTCCGACT
      481 CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA AAATAAGGTT ATCAAGTGAG
      541 AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA AGAGTTTATG CATTTCTTTC
      601 CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA AATCACTCGC ACCAACCAAA
      661 CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA CGCGATCGCC GTTAAAAGGA
      721 CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGAACA CTGCCAGCGC ATCAACAATA
      781 TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG CTGTTTTCCC TGGGATCGCA
      841 GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT GCTTGATGGT CGGAAGAGGC
      901 ATAAATTCCG TCAGCCAGTT TAGCCTGACC ATCTCATCTG TAACATCATT GGCAACGCTA
      961 CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT TCCCATACAA TCGATAGATT
     1021 GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT ACCCATATAA ATCAGCATCC
     1081 ATGTTGGAAT TTAATCGCGG CTTCAAGCAA GACGTTTCCC GTTGAATATG GCTCATTTTA
     1141 GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT
     1201 TCATTATGGT GAAAgttgga acctcttacg tgccGATCAA GTCAAAAGCC TCCGGTCGGA
     1261 GGCTTTTGAC TTTCTGCTAT GGAGGTCAGG TATGATTTtt GAATTCGCTC TTCgATGAGA
     1321 GACCgcaatG GATCCaacca ttGGTCTCAG GTAGAAGAGC AAGCTTGGAT CGACGAGAGC
     1381 AGCGCGACTG GATCTGTCGC CCGTCTCAAA CGCAACCCTC CGGCGGTCGC ATATCATTCA
     1441 GGACGAGCCT CAGACTCCAG CGTAACTGGA CTGCAATCAA CTCACTGGCT CACCTTCCGG
     1501 TCCACGATCA GCTAGAATCA AGCTGACTAG ATAAACTGGC CGTCGTTTTA CACGGGTGGG
     1561 CCTTTCTTCG GTAGAAAATC AAAGGATCTT Cttgagatcc tttttttctg cgcgtaatct
     1621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1681 taccaactct ttttccgagg taactggctt cagcagagcg cagataccaa atactgttct
     1741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1921 g     
//
