LOCUS       DR274_TIR1_SAP__        3770 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >DR274 sequence 2147 bps
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1680..2654
                     /locus_tag="sequenced"
                     /label="sequenced"
                     /ApEinfo_label="sequenced"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3749..3766)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..3563
                     /locus_tag="optimized TIR1 D170E_M473L "
                     /label="optimized TIR1 D170E_M473L "
                     /ApEinfo_label="optimized TIR1 D170E_M473L "
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3564..3566
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1672..1678
                     /locus_tag="SapI"
                     /label="SapI"
                     /ApEinfo_label="SapI"
                     /ApEinfo_fwdcolor="#ea17ff"
                     /ApEinfo_revcolor="#ea17ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3568..3575
                     /locus_tag="SapI(1)"
                     /label="SapI(1)"
                     /ApEinfo_label="SapI"
                     /ApEinfo_fwdcolor="#ea17ff"
                     /ApEinfo_revcolor="#ea17ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3564..3566
                     /locus_tag="AAG Overlap"
                     /label="AAG Overlap"
                     /ApEinfo_label="AAG Overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGCAAAAGAG AATCGCCTTG TCGTTTCCAG AAGAAGTACT CGAGCACGTC TTCTCCTTCA
     1741 TCCAACTCGA CAAGGACCGT AACTCCGTCT CCCTCGTCTG CAAGTCCTGG TACGAGATCG
     1801 AGCGTTGGTG CCGTCGTAAG GTCTTCATCG GAAACTGCTA CGCCGTCTCC CCAGCCACCG
     1861 TCATCCGTCG TTTCCCAAAG GTCCGTTCCG TCGAGCTCAA GGGAAAGCCA CACTTCGCCG
     1921 ACTTCAACCT CGTCCCAGAC GGATGGGGAG GATACGTCTA CCCATGGATC GAGGCCATGT
     1981 CCTCCTCCTA CACCTGGCTC GAGGAGATCC GTCTCAAGCG TATGGTCGTC ACCGACGACT
     2041 GCCTCGAGCT CATCGCCAAG TCCTTCAAGA ACTTCAAGGT CCTCGTCCTC TCCTCCTGCG
     2101 AGGGATTCTC CACCGACGGA CTCGCCGCCA TCGCCGCCAC CTGCCGTAAC CTCAAGgtaa
     2161 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagGAG CTCGACCTCC
     2221 GTGAGTCCGA CGTCGACGAG GTCTCCGGAC ACTGGCTCTC CCACTTCCCA GACACCTACA
     2281 CCTCCCTCGT CTCCCTCAAC ATCTCCTGCC TCGCCTCCGA GGTCTCCTTC TCCGCCCTCG
     2341 AGCGTCTCGT CACCCGTTGC CCAAACCTCA AGTCCCTCAA GCTCAACCGT GCCGTCCCAC
     2401 TCGAGAAGCT CGCCACCCTC CTCCAACGTG CCCCACAACT CGAGGAGCTC GGAACCGGAG
     2461 GATACACCGC CGAGGTCCGT CCAGACGTCT ACTCCGGACT CTCCGTCGCC CTCTCCGGAT
     2521 GCAAGgtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGAGC
     2581 TCCGTTGCCT CTCCGGATTC TGGGACGCCG TCCCAGCCTA CCTCCCAGCC GTCTACTCCG
     2641 TCTGCTCCCG TCTCACCACC CTCAACCTCT CCTACGCCAC CGTCCAATCC TACGACCTCG
     2701 TCAAGCTCCT CTGCCAATGC CCAAAGCTCC AACGTCTCTG GGTCCTCGAC TACATCGAGG
     2761 ACGCCGGACT CGAGGTCCTC GCCTCCACCT GCAAGGACCT CCGTGAGCTC CGTGTCTTCC
     2821 CATCCGAGCC ATTCGTCATG GAGCCAAACG TCGCCCTCAC CGAGCAAGGA CTCGTCTCCG
     2881 TCTCCATGGG ATGCCCAAAG CTCGAGTCCG TCCTCTACTT CTGCCGTCAA ATGACCAACG
     2941 CCGCCCTCAT CACCATCGCC CGTAACCGTC CAAACATGAC CCGTTTCCGT CTCTGCATCA
     3001 TCGAGCCAAA GGCCCCAGAC TACCTCACCC TCGAGCCACT CGACATCGGA TTCGGAGCCA
     3061 TCGTCGAGCA CTGCAAGGAC CTCCGTCGTC TCTCCCTCTC CGGACTCCTC ACCGACAAGG
     3121 TCTTCGAGTA CATCGGAACC TACGCCAAGA AGATGGAGAT GCTCTCCGTC GCCTTCGCCG
     3181 GAGACTCCGA CCTCGGACTC CACCACGTCC TCTCCGGATG CGACTCCCTC CGTAAGCTCG
     3241 AGATCCGTGA CTGCCCATTC GGAGACAAGG CCCTCCTCGC CAACGCCTCC AAGCTCGAGA
     3301 CCATGCGTTC CCTCTGGATG TCCTCCTGCT CCGTCTCCTT CGGAGCCTGC AAGCTCCTCG
     3361 GACAAAAGAT GCCAAAGCTC AACGTCGAGG TCATCGACGA GCGTGGAGCC CCAGACTCCC
     3421 GTCCAGAGTC CTGCCCAGTC GAGCGTGTCT TCATCTACCG TACCGTCGCC GGACCACGTT
     3481 TCGACATGCC AGGATTCGTC TGGAACATGG ACCAAGACTC CACCATGCGT TTCTCCCGTC
     3541 AAATCATCAC CACCAACGGA CTCAAGcGAA GAGCAAGCTT GGATCGACGA GAGCAGCGCG
     3601 ACTGGATCTG TCGCCCGTCT CAAACGCAAC CCTCCGGCGG TCGCATATCA TTCAGGACGA
     3661 GCCTCAGACT CCAGCGTAAC TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG
     3721 ATCAGCTAGA ATCAAGCTGA CTAGATAAAC TGGCCGTCGT TTTACACGGG 
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