LOCUS       DR274_FP_Tomato_        3317 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1645..3075
                     /locus_tag="tdTomato"
                     /label="tdTomato"
                     /ApEinfo_label="tdTomato"
                     /ApEinfo_fwdcolor="#ff4646"
                     /ApEinfo_revcolor="#ff4646"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3261..3278)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(651..1466)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1637..1643
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3080..3086
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..588)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3076..3078
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="#80ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1545..1564
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt aactggcttc
      121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaAA CGCCAGCAAC
      601 GCAGAAAGGC CCACCCGAAG GTGAGCCAGG TGATTACATT TAGGTCCTCA TTAGAAAAAC
      661 TCATCGAGCA TCAAGTGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT
      721 TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA
      781 AGATCCTGGT ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC
      841 CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT
      901 GAGAATGGCA AGAGTTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC
      961 TCGTCATCAA AATCACTCGC ACCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG
     1021 AGACGAAATA CGCGATCGCC GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG
     1081 CGCAGGAACA CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT
     1141 ACCTGGAATG CTGTTTTCCC TGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA
     1201 CGGATAAAAT GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGCCTGACC
     1261 ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC
     1321 GCATCGGGCT TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA
     1381 GCCCATTTAT ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CTTCAAGCAA
     1441 GACGTTTCCC GTTGAATATG GCTCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1501 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAgttgga acctcttacg
     1561 tgccGATCAA GTCAAAAGCC TCCGGTCGGA GGCTTTTGAC TTTCTGCTAT GGAGGTCAGG
     1621 TATGATTTtt GAATTCGCTC TTCgATGGTG AGCAAGGGCG AGGAGGTCAT CAAAGAGTTC
     1681 ATGCGCTTCA AGGTGCGCAT GGAGGGCTCC ATGAACGGCC ACGAGTTCGA GATCGAGGGC
     1741 GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGC
     1801 GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCCCAGT TCATGTACGG CTCCAAGGCG
     1861 TACGTGAAGC ACCCCGCCGA CATCCCCGAT TACAAGAAGC TGTCCTTCCC CGAGGGCTTC
     1921 AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGTCTGG TGACCGTGAC CCAGGACTCC
     1981 TCCCTGCAGG ACGGCACGCT GATCTACAAG GTGAAGATGC GCGGCACCAA CTTCCCCCCC
     2041 GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA GCGCCTGTAC
     2101 CCCCGCGACG GCGTGCTGAA GGGCGAGATC CACCAGGCCC TGAAGCTGAA GGACGGCGGC
     2161 CACTACCTGG TGGAGTTCAA GACCATCTAC ATGGCCAAGA AGCCCGTGCA ACTGCCCGGC
     2221 TACTACTACG TGGACACCAA GCTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG
     2281 GAACAGTACG AGCGCTCCGA GGGCCGCCAC CACCTGTTCC TGGGGCATGG CACCGGCAGC
     2341 ACCGGCAGCG GCAGCTCCGG CACCGCCTCC TCCGAGGACA ACAACATGGC CGTCATCAAA
     2401 GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCATGA ACGGCCACGA GTTCGAGATC
     2461 GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC
     2521 AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CCCAGTTCAT GTACGGCTCC
     2581 AAGGCGTACG TGAAGCACCC CGCCGACATC CCCGATTACA AGAAGCTGTC CTTCCCCGAG
     2641 GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GTCTGGTGAC CGTGACCCAG
     2701 GACTCCTCCC TGCAGGACGG CACGCTGATC TACAAGGTGA AGATGCGCGG CACCAACTTC
     2761 CCCCCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CACCGAGCGC
     2821 CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGATCCACC AGGCCCTGAA GCTGAAGGAC
     2881 GGCGGCCACT ACCTGGTGGA GTTCAAGACC ATCTACATGG CCAAGAAGCC CGTGCAACTG
     2941 CCCGGCTACT ACTACGTGGA CACCAAGCTG GACATCACCT CCCACAACGA GGACTACACC
     3001 ATCGTGGAAC AGTACGAGCG CTCCGAGGGC CGCCACCACC TGTTCCTGTA CGGCATGGAC
     3061 GAGCTGTACA AGTACAAGgG AAGAGCAAGC TTGGATCGAC GAGAGCAGCG CGACTGGATC
     3121 TGTCGCCCGT CTCAAACGCA ACCCTCCGGC GGTCGCATAT CATTCAGGAC GAGCCTCAGA
     3181 CTCCAGCGTA ACTGGACTGC AATCAACTCA CTGGCTCACC TTCCGGTCCA CGATCAGCTA
     3241 GAATCAAGCT GACTAGATAA ACTGGCCGTC GTTTTACACG GGTGGGCCTT TCTTCGGTAG
     3301 AAAATCAAAG GATCTTC    
//
