LOCUS       DR274_AAG_GTA_tb        2222 bp    DNA        circular     03-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >DR274 sequence 2147 bps
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2201..2218)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           1686..2018
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..1682
                     /locus_tag="SAPI overhang"
                     /label="SAPI overhang"
                     /ApEinfo_label="SAPI overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2016..2018
                     /locus_tag="SAPI overhang(1)"
                     /label="SAPI overhang(1)"
                     /ApEinfo_label="SAPI overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCGA
     1681 AGTAAtaaat gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt
     1741 tgtcaaaaaa ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt
     1801 tatagtcact gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgtc
     1861 tgatgtactt tcacaatctc tcttcaattc cattttgaag tgctttaaac ccgaaaggtt
     1921 gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga
     1981 ggaaacttta ttgtgccgcc aagaaaaaag tctcaGTACG AAGAGCAAGC TTGGATCGAC
     2041 GAGAGCAGCG CGACTGGATC TGTCGCCCGT CTCAAACGCA ACCCTCCGGC GGTCGCATAT
     2101 CATTCAGGAC GAGCCTCAGA CTCCAGCGTA ACTGGACTGC AATCAACTCA CTGGCTCACC
     2161 TTCCGGTCCA CGATCAGCTA GAATCAAGCT GACTAGATAA ACTGGCCGTC GTTTTACACG
     2221 GG     
//
