LOCUS       DR274_AAG_GTA_le        2300 bp    DNA        circular     03-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    8..145
                     /locus_tag="let-858 3'UTR"
                     /label="let-858 3'UTR"
                     /ApEinfo_label="let-858 3'UTR"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(601..618)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2202..2221
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    146..415
                     /locus_tag="let-858 flanking (polyA included)"
                     /label="let-858 flanking (polyA included)"
                     /ApEinfo_label="let-858 flanking (polyA included)"
                     /ApEinfo_fwdcolor="#ffcc66"
                     /ApEinfo_revcolor="#ffcc66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1308..2123)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2..4
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(658..1245)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    420..426
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gAAGTGAatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
       61 tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt
      121 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
      181 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
      241 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
      301 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa
      361 ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttggGTAgG
      421 AAGAGCAAGC TTGGATCGAC GAGAGCAGCG CGACTGGATC TGTCGCCCGT CTCAAACGCA
      481 ACCCTCCGGC GGTCGCATAT CATTCAGGAC GAGCCTCAGA CTCCAGCGTA ACTGGACTGC
      541 AATCAACTCA CTGGCTCACC TTCCGGTCCA CGATCAGCTA GAATCAAGCT GACTAGATAA
      601 ACTGGCCGTC GTTTTACACG GGTGGGCCTT TCTTCGGTAG AAAATCAAAG GATCTTCttg
      661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
      781 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      961 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1081 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1141 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1201 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaAACGC CAGCAACGCA
     1261 GAAAGGCCCA CCCGAAGGTG AGCCAGGTGA TTACATTTAG GTCCTCATTA GAAAAACTCA
     1321 TCGAGCATCA AGTGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA
     1381 AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA
     1441 TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC
     1501 TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG
     1561 AATGGCAAGA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG
     1621 TCATCAAAAT CACTCGCACC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA
     1681 CGAAATACGC GATCGCCGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC
     1741 AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC
     1801 TGGAATGCTG TTTTCCCTGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG
     1861 ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG CCTGACCATC
     1921 TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA
     1981 TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC
     2041 CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCTT CAAGCAAGAC
     2101 GTTTCCCGTT GAATATGGCT CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA
     2161 AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA Agttggaacc tcttacgtgc
     2221 cGATCAAGTC AAAAGCCTCC GGTCGGAGGC TTTTGACTTT CTGCTATGGA GGTCAGGTAT
     2281 GATTTttGAA TTCGCTCTTC    
//
