LOCUS       DR274_mec_7p_bas        2040 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     102..114
                     /locus_tag="NMoligo 3218"
                     /label="NMoligo 3218"
                     /ApEinfo_label="NMoligo 3218"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(440..457)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    93..99
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     192..211
                     /locus_tag="NMoligo 3436"
                     /label="NMoligo 3436"
                     /ApEinfo_label="NMoligo 3436"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1147..1962)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(234..253)
                     /locus_tag="NMoligo 3706"
                     /label="NMoligo 3706"
                     /ApEinfo_label="NMoligo 3706"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(497..1084)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(234..253)
                     /locus_tag="NMoligo 4273"
                     /label="NMoligo 4273"
                     /ApEinfo_label="NMoligo 4273"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1..20
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    259..265
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..257
                     /locus_tag="mec-7 basal promoter"
                     /label="mec-7 basal promoter"
                     /ApEinfo_label="mec-7 basal promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gttggaacct cttacgtgcc GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
       61 TGCTATGGAG GTCAGGTATG ATTTttGAAT TCGCTCTTCg GCGagatgtg actcctccca
      121 ttattcgtct tgagtcgcat cttttgtctt ggtccgttgt cagtcgagcc tcaaggcagc
      181 cgttccgcca gtgatcgcct gctaagaagt tgtaggtgta gaggaagaaa cgtcgggtcc
      241 aaatttcaag caacATGaGA AGAGCAAGCT TGGATCGACG AGAGCAGCGC GACTGGATCT
      301 GTCGCCCGTC TCAAACGCAA CCCTCCGGCG GTCGCATATC ATTCAGGACG AGCCTCAGAC
      361 TCCAGCGTAA CTGGACTGCA ATCAACTCAC TGGCTCACCT TCCGGTCCAC GATCAGCTAG
      421 AATCAAGCTG ACTAGATAAA CTGGCCGTCG TTTTACACGG GTGGGCCTTT CTTCGGTAGA
      481 AAATCAAAGG ATCTTCttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      541 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      601 cgaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1021 tcgccacctc tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1081 gaaaAACGCC AGCAACGCAG AAAGGCCCAC CCGAAGGTGA GCCAGGTGAT TACATTTAGG
     1141 TCCTCATTAG AAAAACTCAT CGAGCATCAA GTGAAACTGC AATTTATTCA TATCAGGATT
     1201 ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA
     1261 GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT
     1321 ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT
     1381 GACGACTGAA TCCGGTGAGA ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC
     1441 AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG
     1501 TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG
     1561 AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC
     1621 AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA
     1681 TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG
     1741 CCAGTTTAGC CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT
     1801 CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG
     1861 CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA
     1921 TCGCGGCTTC AAGCAAGACG TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC
     1981 TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA
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