LOCUS       DR274_NT_GFP_C1_        2756 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(1683..1988,2037..2111,2163..2282,2334..2549)
                     /locus_tag="GFP-C1"
                     /label="GFP-C1"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#00ff33"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgG
     1681 GTATGGTCAG TAAAGGAGAA GAATTGTTCA CTGGAGTTGT CCCAATCCTC GTCGAGCTCG
     1741 ACGGAGACGT CAACGGACAC AAGTTCTCCG TCTCCGGAGA GGGAGAGGGA GACGCCACCT
     1801 ACGGAAAGCT CACCCTCAAG TTCATCTGCA CCACCGGAAA GCTCCCAGTC CCATGGCCAA
     1861 CCCTCGTCAC CACCTTCTGC TACGGAGTCC AATGCTTCTC CCGTTACCCA GACCACATGA
     1921 AGCGTCACGA CTTCTTCAAG TCCGCCATGC CAGAGGGATA CGTCCAAGAG CGTACCATCT
     1981 TCTTCAAGgt aagtttaaac atatatatac taactactga ttatttaaat tttcagGACG
     2041 ACGGAAACTA CAAGACCCGT GCCGAGGTCA AGTTCGAGGG AGACACCCTC GTCAACCGTA
     2101 TCGAGCTCAA Ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     2161 agGGAATCGA CTTCAAGGAG GACGGAAACA TCCTCGGACA CAAGCTCGAG TACAACTACA
     2221 ACTCCCACAA CGTCTACATC ATGGCCGACA AGCAAAAGAA CGGAATCAAG GTCAACTTCA
     2281 AGgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagATCCGTC
     2341 ACAACATCGA GGACGGATCC GTCCAACTCG CCGACCACTA CCAACAAAAC ACCCCAATCG
     2401 GAGACGGACC AGTCCTCCTC CCAGACAACC ACTACCTCTC CACCCAATCC GCCCTCTCCA
     2461 AGGACCCAAA CGAGAAGCGT GACCACATGG TCCTCCTCGA GTTCGTCACC GCCGCCGGAA
     2521 TCACCCACGG AATGGACGAG CTCTACAAGA CGcGAAGAGC AAGCTTGGAT CGACGAGAGC
     2581 AGCGCGACTG GATCTGTCGC CCGTCTCAAA CGCAACCCTC CGGCGGTCGC ATATCATTCA
     2641 GGACGAGCCT CAGACTCCAG CGTAACTGGA CTGCAATCAA CTCACTGGCT CACCTTCCGG
     2701 TCCACGATCA GCTAGAATCA AGCTGACTAG ATAAACTGGC CGTCGTTTTA CACGGG
//
