LOCUS       DR274_NT_scarlet        2738 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1627..2555
                     /locus_tag="sequenced 5008"
                     /label="sequenced 5008"
                     /ApEinfo_label="sequenced 5008"
                     /ApEinfo_fwdcolor="#929292"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2717..2734)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(1683..1835,1887..2099,2151..2321,2373..2531)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1683..1685
                     /locus_tag="ATG Overlap"
                     /label="ATG Overlap"
                     /ApEinfo_label="ATG Overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCGG
     1681 GTATGGTTTC CAAGGGAGAG GCCGTCATCA AGGAGTTCAT GCGTTTCAAG GTCCACATGG
     1741 AGGGATCCAT GAACGGACAC GAGTTCGAGA TCGAGGGAGA GGGAGAGGGA CGTCCATACG
     1801 AGGGAACCCA AACCGCCAAG CTCAAGGTCA CCAAGgtaag tttaaacata tatatactaa
     1861 ctaaccctga ttatttaaat tttcagGGAG GACCACTCCC ATTCTCCTGG GACATCCTCT
     1921 CCCCACAATT CATGTACGGA TCCCGTGCCT TCATCAAGCA CCCAGCCGAC ATCCCAGACT
     1981 ACTACAAGCA ATCCTTCCCA GAGGGATTCA AGTGGGAGCG TGTCATGAAC TTCGAGGACG
     2041 GAGGAGCCGT CACCGTCACC CAAGACACCT CCCTCGAGGA CGGAACCCTC ATCTACAAGg
     2101 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag GTCAAGCTCC
     2161 GTGGAACCAA CTTCCCACCA GACGGACCAG TCATGCAAAA GAAGACCATG GGATGGGAGG
     2221 CCTCCACCGA GCGTCTCTAC CCAGAGGACG GAGTCCTCAA GGGAGACATC AAGATGGCCC
     2281 TCCGTCTCAA GGACGGAGGA CGTTACCTCG CCGACTTCAA Ggtaagttta aacatgattt
     2341 tactaactaa ctaatctgat ttaaattttc agACCACCTA CAAGGCCAAG AAGCCAGTCC
     2401 AAATGCCAGG AGCCTACAAC GTCGACCGTA AGCTCGACAT CACCTCCCAC AACGAGGACT
     2461 ACACCGTCGT CGAGCAATAC GAGCGTTCCG AGGGACGTCA CTCCACCGGA GGAATGGACG
     2521 AGCTCTACAA GACGcGAAGA GCAAGCTTGG ATCGACGAGA GCAGCGCGAC TGGATCTGTC
     2581 GCCCGTCTCA AACGCAACCC TCCGGCGGTC GCATATCATT CAGGACGAGC CTCAGACTCC
     2641 AGCGTAACTG GACTGCAATC AACTCACTGG CTCACCTTCC GGTCCACGAT CAGCTAGAAT
     2701 CAAGCTGACT AGATAAACTG GCCGTCGTTT TACACGGG  
//
