LOCUS       DR274_FP_tTAiS_L        3140 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(651..1466)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3084..3101)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2230..2232
                     /locus_tag="rtTA-Q"
                     /label="rtTA-Q"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2626..2898
                     /locus_tag="sequenced 6735"
                     /label="sequenced 6735"
                     /ApEinfo_label="sequenced 6735"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2353..2394
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2136..2229
                     /locus_tag="rtTA-Q(1)"
                     /label="rtTA-Q(1)"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2903..2909
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2233..2319
                     /locus_tag="rtTA-Q(2)"
                     /label="rtTA-Q(2)"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2880..2898)
                     /locus_tag="NMoligo 6724"
                     /label="NMoligo 6724"
                     /ApEinfo_label="NMoligo 6724"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2233..2352
                     /locus_tag="sequenced 4618"
                     /label="sequenced 4618"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     primer_bind     complement(2877..2895)
                     /locus_tag="NMoligo 6734"
                     /label="NMoligo 6734"
                     /ApEinfo_label="NMoligo 6734"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..588)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2230..2232
                     /locus_tag="rTetR"
                     /label="rTetR"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2351..2352
                     /locus_tag="NMoligo 6723"
                     /label="NMoligo 6723"
                     /ApEinfo_label="NMoligo 6723"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2136..2229
                     /locus_tag="rTetR(1)"
                     /label="rTetR(1)"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2395..2898
                     /locus_tag="QF activation domain"
                     /label="QF activation domain"
                     /ApEinfo_label="QF activation domain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1545..1564
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2230..2232
                     /locus_tag="sequenced 4618(1)"
                     /label="sequenced 4618(1)"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2136..2229
                     /locus_tag="sequenced 4618(2)"
                     /label="sequenced 4618(2)"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2233..2319
                     /locus_tag="rTetR(2)"
                     /label="rTetR(2)"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1637..1643
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2230..2232
                     /locus_tag="R TO T VARIENT arg in rtTA his in tTA "
                     /label="R TO T VARIENT arg in rtTA his in tTA "
                     /ApEinfo_label="R TO T VARIENT arg in rtTA his in tTA "
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1645..2135
                     /locus_tag="tTA"
                     /label="tTA"
                     /ApEinfo_label="tTA"
                     /ApEinfo_fwdcolor="#0096ff"
                     /ApEinfo_revcolor="#0096ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2323..2352
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#a9a9a9"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2880..2898)
                     /locus_tag="NMoligo 6711"
                     /label="NMoligo 6711"
                     /ApEinfo_label="NMoligo 6711"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2206..2208
                     /locus_tag="R TO T VARIENT lys in rtTA Arg in tTA"
                     /label="R TO T VARIENT lys in rtTA Arg in tTA"
                     /ApEinfo_label="R TO T VARIENT lys in rtTA Arg in tTA"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2137..2139
                     /locus_tag="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /label="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /ApEinfo_label="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1977..1977
                     /locus_tag="introduced stop in intron"
                     /label="introduced stop in intron"
                     /ApEinfo_label="introduced stop in intron"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1979..1979
                     /locus_tag="introduces stop in intron"
                     /label="introduces stop in intron"
                     /ApEinfo_label="introduces stop in intron"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1633..2352
                     /locus_tag="sequenced 5008"
                     /label="sequenced 5008"
                     /ApEinfo_label="sequenced 5008"
                     /ApEinfo_fwdcolor="#797979"
                     /ApEinfo_revcolor="#797979"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2695..2914
                     /locus_tag="sequenced 6133"
                     /label="sequenced 6133"
                     /ApEinfo_label="sequenced 6133"
                     /ApEinfo_fwdcolor="#797979"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2395..2625
                     /locus_tag="sequenced 4618(3)"
                     /label="sequenced 4618(3)"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     primer_bind     2395..2411
                     /locus_tag="NMoligo 6723(1)"
                     /label="NMoligo 6723(1)"
                     /ApEinfo_label="NMoligo 6723"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2395..2643
                     /locus_tag="sequenced 5008(1)"
                     /label="sequenced 5008(1)"
                     /ApEinfo_label="sequenced 5008"
                     /ApEinfo_fwdcolor="#797979"
                     /ApEinfo_revcolor="#797979"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt aactggcttc
      121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaAA CGCCAGCAAC
      601 GCAGAAAGGC CCACCCGAAG GTGAGCCAGG TGATTACATT TAGGTCCTCA TTAGAAAAAC
      661 TCATCGAGCA TCAAGTGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT
      721 TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA
      781 AGATCCTGGT ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC
      841 CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT
      901 GAGAATGGCA AGAGTTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC
      961 TCGTCATCAA AATCACTCGC ACCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG
     1021 AGACGAAATA CGCGATCGCC GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG
     1081 CGCAGGAACA CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT
     1141 ACCTGGAATG CTGTTTTCCC TGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA
     1201 CGGATAAAAT GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGCCTGACC
     1261 ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC
     1321 GCATCGGGCT TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA
     1381 GCCCATTTAT ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CTTCAAGCAA
     1441 GACGTTTCCC GTTGAATATG GCTCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC
     1501 TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAgttgga acctcttacg
     1561 tgccGATCAA GTCAAAAGCC TCCGGTCGGA GGCTTTTGAC TTTCTGCTAT GGAGGTCAGG
     1621 TATGATTTtt GAATTCGCTC TTCgATGTCA CGTCTAGATA AATCCAAGGT CATCAACTCC
     1681 GCCCTCGAGC TCCTCAATGA GGTCGGAATT GAGGGACTCA CCACCCGTAA GCTCGCCCAA
     1741 AAGCTCGGAG TTGAGCAACC AACCCTCTAC TGGCACGTCA AGAACAAGCG TGCACTTCTC
     1801 GACGCCCTCG CCATCGAGAT GCTCGATCGT CACCACACCC ACTTCTGCCC ACTCGAGGGA
     1861 GAATCCTGGC AAGATTTCCT CCGTAACAAC GCCAAGTCCT TCCGTTGCGC CCTTCTCTCC
     1921 CACCGTGACG GAGCCAAGgt aagtttaaac atatatatac taactaaccc tgattaatga
     1981 aattttcagG TCCATCTCGG AACCCGTCCA ACCGAGAAGC AATACGAGAC TCTCGAGAAC
     2041 CAACTTGCCT TCTTGTGCCA ACAAGGATTC TCCCTCGAAA ACGCCCTCTA CGCCCTCTCC
     2101 GCCGTCGGAC ATTTCACCCT CGGATGCGTC CTCGAAGATC AAGAACATCA AGTTGCAAAA
     2161 GAAGAACGAG AAACTCCAAC AACTGATTCA ATGCCACCAC TTTTGAGACA AGCTATCGAA
     2221 CTTTTCGATC ACCAAGGAGC AGAACCAGCT TTCCTTTTTG GACTGGAACT TATTATCTGT
     2281 GGACTTGAGA AACAACTGAA ATGTGAATCG GGAGGAGGAG CGGGTTCTGG ATCTGGATCT
     2341 GGAGGTTCCG GTGGTGGCAG CGCTGGAGGT ACCGGCGGTA GTGCCGGAAC GGGTCGTCAA
     2401 CTTGAGCTCC CACCAACCGC CACCGCCACC GCCAGTATCA TGCCACACGT CATGGAGCAA
     2461 CCACTCTCCA CCTCCATCAA CCCAGTCAAC GACCGTTTCA ACGGAATACC AAACCCAACC
     2521 CCATACAACT CCGACGCCGC CCTCGACGCC ATCACCCAAA CCAACGACTA CGGATCCGTC
     2581 AACACCCACG GAATCCTCTC CACCTACCCA CCACCAGCCA CCCACCTCAA CGAGGCCTCC
     2641 GTCGCCCTCG CACCAGGAGG AGCACCACCA CGTCCACCAC CACCATACGT CGACTCCACC
     2701 ACCAACCACC CACCATACCA TTCCAACCTC GTCCCAATGG CCAACTTCGG ATACTCCACC
     2761 GTCGACTACG ACGCCATGGT CGACGACCTC GCCTCCATCG AGTACACCGA CGCCGTCGAC
     2821 GTCGACCCAC AATTCATGAC CAACCTCGGG TTCGTCCCAG GATGCAACTT CTCCGACATC
     2881 AACACCTATG AGCAATAAAA GgGAAGAGCA AGCTTGGATC GACGAGAGCA GCGCGACTGG
     2941 ATCTGTCGCC CGTCTCAAAC GCAACCCTCC GGCGGTCGCA TATCATTCAG GACGAGCCTC
     3001 AGACTCCAGC GTAACTGGAC TGCAATCAAC TCACTGGCTC ACCTTCCGGT CCACGATCAG
     3061 CTAGAATCAA GCTGACTAGA TAAACTGGCC GTCGTTTTAC ACGGGTGGGC CTTTCTTCGG
     3121 TAGAAAATCA AAGGATCTTC    
//
