LOCUS       DR274_FP_nlscyOF        2866 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >DR274 sequence 2147 bps
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1680..1682
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1760..1960
                     /locus_tag="CyOFP1"
                     /label="CyOFP1"
                     /ApEinfo_label="CyOFP1"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2222..2386
                     /locus_tag="CyOFP1(1)"
                     /label="CyOFP1(1)"
                     /ApEinfo_label="CyOFP1"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2664..2670
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1683..1688
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     sig_peptide     1689..1709
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1710..1715
                     /locus_tag="CyOFP1(2)"
                     /label="CyOFP1(2)"
                     /ApEinfo_label="CyOFP1"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1716..1759
                     /locus_tag="intron rpl-18"
                     /label="intron rpl-18"
                     /ApEinfo_label="intron rpl-18"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          1961..2011
                     /locus_tag="syntron 3GG"
                     /label="syntron 3GG"
                     /ApEinfo_label="syntron 3GG"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2012..2170
                     /locus_tag="CyOFP1(3)"
                     /label="CyOFP1(3)"
                     /ApEinfo_label="CyOFP1"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2171..2221
                     /locus_tag="syntron GG4 (modified)"
                     /label="syntron GG4 (modified)"
                     /ApEinfo_label="syntron GG4 (modified)"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          2387..2437
                     /locus_tag="syntron 5 GG (modified)"
                     /label="syntron 5 GG (modified)"
                     /ApEinfo_label="syntron 5 GG (modified)"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2438..2602
                     /locus_tag="CyOFP1(4)"
                     /label="CyOFP1(4)"
                     /ApEinfo_label="CyOFP1"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 2603..2662
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGGGATCCCC AAAGAAGAAG CGTAAGGTGT CCAAGgtaac acttagtttt tgttgctctg
     1741 tttaaaaatt aattttaagG GAGAGGAGCT CATCAAGGAG AACATGCGTT CCAAGCTCTA
     1801 CCTCGAGGGA TCCGTCAACG GACACCAATT CAAGTGCACC CACGAGGGAG AGGGAAAGCC
     1861 ATACGAGGGA AAGCAAACCA ACCGTATCAA GGTCGTCGAG GGAGGACCAC TCCCATTCGC
     1921 CTTCGACATC CTCGCCACCC ACTTCATGTA CGGATCCAAG gtaagttttc ttgagacctt
     1981 tactaactaa ctaggtctca tagattttca gGTCTTCATC AAGTACCCAG CCGACCTCCC
     2041 AGACTACTTC AAGCAATCCT TCCCAGAGGG ATTCACCTGG GAGCGTGTCA TGGTCTTCGA
     2101 GGACGGAGGA GTCCTCACCG CCACCCAAGA CACCTCCCTC CAAGACGGAG AGCTCATCTA
     2161 CAACGTCAAG gtaagttatg tagagacctt aatactcaac tatggtctca aacattttca
     2221 gGTCCGTGGA GTCAACTTCC CAGCCAACGG ACCAGTCATG CAAAAGAAGA CCCTCGGATG
     2281 GGAGCCATCC ACCGAGACCA TGTACCCAGC CGACGGAGGA CTCGAGGGAC GTTGCGACAA
     2341 GGCCCTCAAG CTCGTCGGAG GAGGACACCT CCACGTCAAC TTCAAGgtaa gttctttaga
     2401 gacctaatca catcaatttg gtctcagtaa ttttcagACC ACCTACAAGT CCAAGAAGCC
     2461 AGTCAAGATG CCAGGAGTCC ACTACGTCGA CCGTCGTCTC GAGCGTATCA AGGAGGCCGA
     2521 CAACGAGACC TACGTCGAGC AATACGAGCA CGCCGTCGCC CGTTACTCCA ACCTCGGAGG
     2581 AGGAATGGAC GAGCTCTACA AGTCCCGTCG TCGTAAGGCC AACCCAACCA AGCTCTCCGA
     2641 GAACGCCAAG AAGCTCGCCA AGgGAAGAGC AAGCTTGGAT CGACGAGAGC AGCGCGACTG
     2701 GATCTGTCGC CCGTCTCAAA CGCAACCCTC CGGCGGTCGC ATATCATTCA GGACGAGCCT
     2761 CAGACTCCAG CGTAACTGGA CTGCAATCAA CTCACTGGCT CACCTTCCGG TCCACGATCA
     2821 GCTAGAATCA AGCTGACTAG ATAAACTGGC CGTCGTTTTA CACGGG 
//
