LOCUS       DR274_NT_nls_Sca        2834 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     DR274 NT nls bsaI nls [4134] from 1741 (BsaI) to 1713 (BsaI) 
COMMENT     scarlet C1 NT PCR from 9 (BsaI) to 278 (BsaI) 
COMMENT     scarlet C1 CT PCR from 8 (BsaI) to 584 (BsaI) 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(1716..1868,1920..2132,2184..2354,2406..2563)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     1692..1715
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2632..2638
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 2568..2627
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgG
     1681 GTATGGGATC CCCAAAGAAG AAGCGTAAGG TGTCGATGGT TTCCAAGGGA GAGGCCGTCA
     1741 TCAAGGAGTT CATGCGTTTC AAGGTCCACA TGGAGGGATC CATGAACGGA CACGAGTTCG
     1801 AGATCGAGGG AGAGGGAGAG GGACGTCCAT ACGAGGGAAC CCAAACCGCC AAGCTCAAGG
     1861 TCACCAAGgt aagtttaaac atatatatac taactaaccc tgattattta aattttcagG
     1921 GAGGACCACT CCCATTCTCC TGGGACATCC TCTCCCCACA ATTCATGTAC GGATCCCGTG
     1981 CCTTCACCAA GCACCCAGCC GACATCCCAG ACTACTACAA GCAATCCTTC CCAGAGGGAT
     2041 TCAAGTGGGA GCGTGTCATG AACTTCGAGG ACGGAGGAGC CGTCACCGTC ACCCAAGACA
     2101 CCTCCCTCGA GGACGGAACC CTCATCTACA AGgtaagttt aaacagttcg gtactaacta
     2161 accatacata tttaaatttt cagGTCAAGC TCCGTGGAAC CAACTTCCCA CCAGACGGAC
     2221 CAGTCATGCA AAAGAAGACC ATGGGATGGG AGGCCTCCAC CGAGCGTCTC TACCCAGAGG
     2281 ACGGAGTCCT CAAGGGAGAC ATCAAGATGG CCCTCCGTCT CAAGGACGGA GGACGTTACC
     2341 TCGCCGACTT CAAGgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     2401 ttcagACCAC CTACAAGGCC AAGAAGCCAG TCCAAATGCC AGGAGCCTAC AACGTCGACC
     2461 GTAAGCTCGA CATCACCTCC CACAACGAGG ACTACACCGT CGTCGAGCAA TACGAGCGTT
     2521 CCGAGGGACG TCACTCCACC GGAGGAATGG ACGAGCTCTA CAAGGCCTCC CGTCGTCGTA
     2581 AGGCCAACCC AACCAAGCTC TCCGAGAACG CCAAGAAGCT CGCCAAGACG cGAAGAGCAA
     2641 GCTTGGATCG ACGAGAGCAG CGCGACTGGA TCTGTCGCCC GTCTCAAACG CAACCCTCCG
     2701 GCGGTCGCAT ATCATTCAGG ACGAGCCTCA GACTCCAGCG TAACTGGACT GCAATCAACT
     2761 CACTGGCTCA CCTTCCGGTC CACGATCAGC TAGAATCAAG CTGACTAGAT AAACTGGCCG
     2821 TCGTTTTACA CGGG    
//
