LOCUS       DR274_FP_tomm_20        2870 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2849..2866)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2668..2674
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(1680..1821,1885..2177,2535..2663)
                     /locus_tag="tomm-20"
                     /label="tomm-20"
                     /ApEinfo_label="tomm-20"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGTCGGACAC AATTCTTGGT TTCAACAAAT CAAACGTCGT TTTGGCTGCT GGAATTGCTG
     1741 GAGCCGCTTT CCTCGGCTAC TGCATTTACT TCGATCATAA GAGAATCAAC GCTCCAGACT
     1801 ACAAGGACAA GATTAGGCAA Agtcagtgtt ttaacaacat atttccttcg gatttttatc
     1861 taaaaacaac ttattttctt tcagAGAGAC GTGCCCAGGC TGGAGCAGGA GGAATGGCTC
     1921 CACGTCGTCC AGCTGCAGCA GGCAATGATG CTGCTCCAGA TGTCACCGAT CCATCCCAAA
     1981 TGCAAAGGTT CTTCTTGCAA GAGGTTCAAC TCGGAGAAGA ACTCATGGCA GCCGGAAATG
     2041 TTGATGAAGG TGCCGTGCAC ATTGCCAACG CAGTCATGCT CTGCGGTGAA TCTCAACAAC
     2101 TCCTGTCTAT CTTCCAACAA ACATTGTCGG AAGATCAGTT CCGTGCTGTT GTTCAACAAT
     2161 TGCCGTCGAC TCGCGAAgta agtggttttt aaatgtaaaa attcacttta aaagttattg
     2221 aaatgtgact gttaaatact atttaaaaaa ttttcatgtg aaaacctggc attcaaatta
     2281 acctgtccga gaaaatattc gaaattaaat atacaatttt gaaggatgca aaattttctt
     2341 ataattttga gccacattcc atcaaaaatg cgttatttca gtacaacagt ttcaaatgca
     2401 aattaaacat tcgaatatct aggagaactt attcaaaatt ctagttaagt aggcatatat
     2461 tcaaactaga attttttact cacagaaatt ggtttatttt ttcaattttc aatatcttct
     2521 atgttaattt ttagCGTCTT GCGGAAATGT TCGGAGCGAA AGCAGATGAG GCAGAAAACG
     2581 AGCCACCAAT GGTTCAATAC CTCGGAGACG GACCTCCACC AGCACAAATC CAAGAGCTTA
     2641 TCGATGACAC CGACGACTTG GAGAAGgGAA GAGCAAGCTT GGATCGACGA GAGCAGCGCG
     2701 ACTGGATCTG TCGCCCGTCT CAAACGCAAC CCTCCGGCGG TCGCATATCA TTCAGGACGA
     2761 GCCTCAGACT CCAGCGTAAC TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG
     2821 ATCAGCTAGA ATCAAGCTGA CTAGATAAAC TGGCCGTCGT TTTACACGGG 
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