LOCUS       DR274_FP_lexA_L_        3173 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     DR274 FP-BsaI [3469] from 1714 (BsaI) to 1677 (BsaI) 
COMMENT     lexA-L-QF PCR from 8 (BsaI) to 1295 (BsaI) 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2433..2462
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#a9a9a9"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(1716..1859,1911..2243,2295..2429)
                     /locus_tag="lexA DB"
                     /label="lexA DB"
                     /ApEinfo_label="lexA DB"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2463..2966
                     /locus_tag="QF activation domain"
                     /label="QF activation domain"
                     /ApEinfo_label="QF activation domain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1683..1715
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..2576
                     /locus_tag="sequenced 4618"
                     /label="sequenced 4618"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2686..2966
                     /locus_tag="sequenced 6735"
                     /label="sequenced 6735"
                     /ApEinfo_label="sequenced 6735"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     primer_bind     complement(3152..3169)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2971..2977
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGCCACCAAA GAAGAAGCGT AAGGTCGAGG ACCCAATGAA GGCCCTCACC GCCCGTCAAC
     1741 AAGAGGTCTT CGACCTCATC CGTGACCACA TCTCCCAAAC CGGAATGCCA CCAACCCGTG
     1801 CCGAGATCGC CCAACGTCTC GGATTCCGTT CCCCAAACGC CGCCGAGGAG CACCTCAAGg
     1861 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag GCTCTCGCTC
     1921 GTAAGGGAGT CATCGAGATC GTCTCCGGAG CCTCCCGTGG AATCCGTCTC CTCCAAGAGG
     1981 AGGAGGAGGG ACTCCCACTC GTCGGACGTG TCGCTGCTGG AGAGCCACTC CTCGCCCAAC
     2041 AACACATCGA GGGACACTAC CAAGTCGACC CATCCCTCTT CAAGCCAAAC GCCGACTTCC
     2101 TCCTCCGTGT CTCCGGAATG TCCATGAAGG ACATCGGAAT CATGGACGGA GATCTCCTCG
     2161 CCGTCCACAA GACCCAAGAC GTCCGTAACG GACAAGTCGT CGTCGCCCGT ATCGACGACG
     2221 AGGTCACCGT CAAGCGTCTT AAGgtaagtt taaacatgat attactaact aactaatctg
     2281 atttaaattt tcagAAGCAA GGAAACAAGG TCGAGCTCCT CCCAGAGAAC TCCGAGTTCA
     2341 AGCCAATCGT CGTCGACCTC CGTCAACAAT CCTTCACCAT CGAGGGACTC GCCGTCGGAG
     2401 TCATCCGTAA CGGAGACTGG CTCGAGCTCG CGGGTTCTGG ATCTGGATCT GGAGGTTCCG
     2461 GTCGTCAACT TGAGCTCCCA CCAACCGCCA CCGCCACCGC CAGTATCATG CCACACGTCA
     2521 TGGAGCAACC ACTCTCCACC TCCATCAACC CAGTCAACGA CCGTTTCAAC GGAATACCAA
     2581 ACCCAACCCC ATACAACTCC GACGCCGCCC TCGACGCCAT CACCCAAACC AACGACTACG
     2641 GATCCGTCAA CACCCACGGA ATCCTCTCCA CCTACCCACC ACCAGCCACC CACCTCAACG
     2701 AGGCCTCCGT CGCCCTCGCA CCAGGAGGAG CACCACCACG TCCACCACCA CCATACGTCG
     2761 ACTCCACCAC CAACCACCCA CCATACCATT CCAACCTCGT CCCAATGGCC AACTTCGGAT
     2821 ACTCCACCGT CGACTACGAC GCCATGGTCG ACGACCTCGC CTCCATCGAG TACACCGACG
     2881 CCGTCGACGT CGACCCACAA TTCATGACCA ACCTCGGGTT CGTCCCAGGA TGCAACTTCT
     2941 CCGACATCAA CACCTATGAG CAATAAAAGg GAAGAGCAAG CTTGGATCGA CGAGAGCAGC
     3001 GCGACTGGAT CTGTCGCCCG TCTCAAACGC AACCCTCCGG CGGTCGCATA TCATTCAGGA
     3061 CGAGCCTCAG ACTCCAGCGT AACTGGACTG CAATCAACTC ACTGGCTCAC CTTCCGGTCC
     3121 ACGATCAGCT AGAATCAAGC TGACTAGATA AACTGGCCGT CGTTTTACAC GGG
//
