LOCUS       DR274_FP_nls_BFP        2846 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Ligation reaction:
COMMENT     DR274 FP tomm-20 [4259] from 2663 (SapI) to 1678 (SapI)
COMMENT     PCR DR274 NT nls-BFP 7070 7071 from 12 (SapI) to 971 (SapI)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..1682
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1683..1688
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2555..2574)
                     /locus_tag="NMoligo 5715"
                     /label="NMoligo 5715"
                     /ApEinfo_label="NMoligo 5715"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1910..1929)
                     /locus_tag="NMoligo 6737"
                     /label="NMoligo 6737"
                     /ApEinfo_label="NMoligo 6737"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2626..2639)
                     /note=") PCR_conditions {primer
                     sequence:ccttGCTCTTCcCTTGGCGAGCTTCTTGG}"
                     /locus_tag="7071"
                     /label="7071"
                     /ApEinfo_label="7071"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(1713..1898,1950..2132,2184..2375,2427..2576)
                     /locus_tag="TagBFP-C1"
                     /label="TagBFP-C1"
                     /ApEinfo_label="TagBFP-C1"
                     /ApEinfo_fwdcolor="#0096ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     1689..1711
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1680..1698
                     /note=") PCR_conditions {primer
                     sequence:TTGTGCTCTTCaATGGGATCCCCAAAGAAGA}"
                     /locus_tag="7070"
                     /label="7070"
                     /ApEinfo_label="7070"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1733..1752
                     /locus_tag="NMoligo 6899"
                     /label="NMoligo 6899"
                     /ApEinfo_label="NMoligo 6899"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2559..2576)
                     /locus_tag="NMoligo 6900"
                     /label="NMoligo 6900"
                     /ApEinfo_label="NMoligo 6900"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1712..1728
                     /locus_tag="NMoligo 6925"
                     /label="NMoligo 6925"
                     /ApEinfo_label="NMoligo 6925"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 2583..2639
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2825..2842)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2644..2650
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGGGATCCCC AAAGAAGAAG CGTAAGGTGT CgATGGTCAG CAAGGGAGAG GAGCTTATTA
     1741 AGGAGAATAT GCATATGAAA CTGTACATGG AAGGAACCGT GGACAACCAT CACTTTAAGT
     1801 GCACTTCGGA GGGGGAAGGC AAACCGTACG AGGGCACCCA GACCATGCGA ATCAAGGTGG
     1861 TCGAGGGAGG CCCACTGCCC TTCGCCTTCG ACATTCTGgt aagtttaaac atatatatac
     1921 taactaaccc tgattattta aattttcagG CTACTAGCTT CCTGTACGGC AGCAAGACTT
     1981 TTATTAATCA CACCCAGGGC ATCCCGGACT TCTTCAAACA GTCGTTCCCT GAGGGCTTCA
     2041 CATGGGAGCG TGTGACCACA TACGAAGACG GTGGCGTTCT CACCGCTACC CAGGACACCA
     2101 GCCTCCAGGA CGGTTGCCTC ATATATAATG TGgtaagttt aaacagttcg gtactaacta
     2161 accatacata tttaaatttt cagAAGATCC GGGGAGTAAA CTTCACATCC AATGGTCCGG
     2221 TTATGCAGAA GAAAACACTC GGCTGGGAGG CCTTCACCGA GACGCTGTAT CCGGCTGACG
     2281 GAGGCCTGGA AGGTCGAAAC GACATGGCCC TGAAGCTCGT CGGCGGTAGC CATCTCATCG
     2341 CAAACATCAA GACCACATAT AGATCCAAGA AACCGgtaag tttaaacatg attttactaa
     2401 ctaactaatc tgatttaaat tttcagGCTA AGAACCTTAA AATGCCGGGA GTCTACTATG
     2461 TTGACTACAG ACTGGAACGG ATCAAGGAGG CCAACAACGA GACTTACGTC GAGCAGCACG
     2521 AGGTTGCAGT TGCCAGATAC TGCGACCTCC CGAGCAAACT GGGTCACAAG CTTAATAAGG
     2581 CCTCCCGTCG TCGTAAGGCC AACCCAACCA AGCTCTCCGA GAACGCCAAG AAGCTCGCCA
     2641 AGgGAAGAGC AAGCTTGGAT CGACGAGAGC AGCGCGACTG GATCTGTCGC CCGTCTCAAA
     2701 CGCAACCCTC CGGCGGTCGC ATATCATTCA GGACGAGCCT CAGACTCCAG CGTAACTGGA
     2761 CTGCAATCAA CTCACTGGCT CACCTTCCGG TCCACGATCA GCTAGAATCA AGCTGACTAG
     2821 ATAAACTGGC CGTCGTTTTA CACGGG   
//
