LOCUS       DR274_CT_NT_gfp1        4363 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(4342..4359)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1680..1718
                     /locus_tag="Flex Linker"
                     /label="Flex Linker"
                     /ApEinfo_label="Flex Linker"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_binding    1797..1844
                     /locus_tag="GFP11"
                     /label="GFP11"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2112..2159
                     /locus_tag="GFP11(1)"
                     /label="GFP11(1)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..439)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4145..4160)
                     /PCR_conditions="primer sequence:cggtctctgcgtgcctccggcact"
                     /locus_tag="New Primer"
                     /label="New Primer"
                     /ApEinfo_label="New Primer"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    1860..1907
                     /locus_tag="GFP11(2)"
                     /label="GFP11(2)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(440..623)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1679..1693
                     /PCR_conditions="primer sequence:tggtctctggcgggcagcggtgg"
                     /locus_tag="New Primer(1)"
                     /label="New Primer(1)"
                     /ApEinfo_label="New Primer"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    1923..1970
                     /locus_tag="GFP11(3)"
                     /label="GFP11(3)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1734..1781
                     /locus_tag="GFP11(4)"
                     /label="GFP11(4)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1986..2033
                     /locus_tag="GFP11(5)"
                     /label="GFP11(5)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..1682
                     /locus_tag="GCG overlap"
                     /label="GCG overlap"
                     /ApEinfo_label="GCG overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2049..2096
                     /locus_tag="GFP11(6)"
                     /label="GFP11(6)"
                     /ApEinfo_label="GFP11"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2175..2177
                     /locus_tag="AAG overlap"
                     /label="AAG overlap"
                     /ApEinfo_label="AAG overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4118..4156
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2248..2264)
                     /PCR_conditions="primer
                     sequence:tggtctcagaccttagaggtcctcctcgg"
                     /locus_tag="New Primer(2)"
                     /label="New Primer(2)"
                     /ApEinfo_label="New Primer"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2265..2291
                     /PCR_conditions="primer
                     sequence:tGGTCTCaGGTCcATTTTTGCTTTCGTCGTAAATC"
                     /locus_tag="New Primer(3)"
                     /label="New Primer(3)"
                     /ApEinfo_label="New Primer"
                     /ApEinfo_fwdcolor="#408000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          2178..2188
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2232..2261
                     /locus_tag="myc"
                     /label="myc"
                     /ApEinfo_label="myc"
                     /ApEinfo_fwdcolor="#6666ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2675..3700
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2223..2228
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2189..2222
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4078..4111
                     /locus_tag="loxP(1)"
                     /label="loxP(1)"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(2269..2269)
                     /locus_tag="hsp16-41 promoter"
                     /label="hsp16-41 promoter"
                     /ApEinfo_label="hsp16-41 promoter"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4112..4117
                     /locus_tag="intron(2)"
                     /label="intron(2)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           3726..4077
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2229..2231
                     /locus_tag="spacer"
                     /label="spacer"
                     /ApEinfo_label="spacer"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2270..2632
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgG
     1681 CGGGCAGCGG TGGCAGTGGA GGTACCGGCG GAAGCGGTAT GGGAGCAGGA TCACGTGACC
     1741 ACATGGTCCT TCATGAGTAT GTgAATGCTG CTGGcATTAC AGGTGGCTCT GGAGGTAGAG
     1801 ATCATATGGT TCTCCACGAA TACGTTAACG CCGCAGGCAT CACTGGCGGT AGTGGAGGAC
     1861 GCGACCATAT GGTcCTACAT GAATATGTCA ATGCAGCCGG AATtACCGGA GGtTCCGGAG
     1921 GCCGtGATCA CATGGTGCTc CATGAGTATG TGAACGCcGC tGGTATcACT GGTGGGTCGG
     1981 GCGGAaGAGA CCATATGGTG CTTCACGAAT ACGTcAACGC AGCTGGCATT ACTGGCGGAT
     2041 CAGGTGGCAG GGATCACATG GTtCTCCATG AGTACGTGAA CGCTGCTGGA ATCACAGGCG
     2101 GTAGCGGCGG TCGtGACCAT ATGGTCtTGC ACGAATATGT CAATGCTGCC GGTATCACCG
     2161 GCGGCAAgTT CATGAAGgta agtttaaaAT AACTTCGTAT AGCATACATT ATACGAAGTT
     2221 ATtttcagGG AGAGCAAAAG CTTATCTCCG AGGAGGACCT CTAAGGTCcA TTTTTGCTTT
     2281 CGTCGTAAAT CTACACACGC GTCTCTTCCG TGCGAGAGTC CAAGCCAGCA GCCAAATTCG
     2341 TTGACTGAGT ATTCAACGTT TATACGTTGT CGGCAACGAG AAATAGGAAA ATGCATCGGG
     2401 AAATGTTCTT TTTTCGATTT TTTCCAAGGT TTTGACAAAT TTTACCACGA ATTTTGCTAT
     2461 GTTTTCAATT AAAAAATATG TTATTCAACT GTTTCTATGA GGAAAATAAG GCTTTGCATG
     2521 TAATTTTCTT ATTCAGCATA ATTTTTAATT AATTTGAATT TTCTGTCCTA ACGTTTATTT
     2581 TGTTTTCTTG GTTATGACTG ATCTGAAATT AATTTTTGAA TTTTAAGGTA ATATGTCAGG
     2641 CGGTGCCGCA AGTTTGTACA AAAAAGCAGG CTCCATGAAA AAGCCTGAAC TCACCGCGAC
     2701 GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC
     2761 GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG
     2821 GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC
     2881 GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA
     2941 TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC
     3001 CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA
     3061 GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA
     3121 TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC
     3181 CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC
     3241 CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG
     3301 CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT
     3361 CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT
     3421 CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC CGGGCGTATA TGCTCCGCAT
     3481 TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC
     3541 GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT
     3601 CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG
     3661 AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGGAATAG ACCCAGCTTT CTTGTACAAA
     3721 GTGGGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT
     3781 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC
     3841 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA
     3901 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT
     3961 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA
     4021 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTAATA
     4081 ACTTCGTATA GCATACATTA TACGAAGTTA TtttcagGGT GGCAGCGCTG GAGGTACCGG
     4141 CGGTAGTGCC GGAGGCACGc GAAGAGCAAG CTTGGATCGA CGAGAGCAGC GCGACTGGAT
     4201 CTGTCGCCCG TCTCAAACGC AACCCTCCGG CGGTCGCATA TCATTCAGGA CGAGCCTCAG
     4261 ACTCCAGCGT AACTGGACTG CAATCAACTC ACTGGCTCAC CTTCCGGTCC ACGATCAGCT
     4321 AGAATCAAGC TGACTAGATA AACTGGCCGT CGTTTTACAC GGG 
//
