LOCUS       DR274_NT_myr_tag        3880 bp    DNA        circular     02-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     DR274 NT-BsaI [3456] from 1714 (BsaI) to 1677 (BsaI) 
COMMENT     tagRFP-T NT PCR from 8 (BsaI) to 2002 (BsaI) 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(1737..1751,1796..2018,2324..2460,2783..2977,
                     3509..3673)
                     /locus_tag="tagRFP-T"
                     /label="tagRFP-T"
                     /ApEinfo_label="tagRFP-T"
                     /ApEinfo_fwdcolor="#cc3333"
                     /ApEinfo_revcolor="#cc3333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1672..1678
                     /locus_tag="SapI>"
                     /label="SapI>"
                     /ApEinfo_label="SapI>"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3859..3876)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3678..3684
                     /locus_tag="<SapI"
                     /label="<SapI"
                     /ApEinfo_label="<SapI"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1683..1736
                     /locus_tag="goa-1 myr signal"
                     /label="goa-1 myr signal"
                     /ApEinfo_label="goa-1 myr signal"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgG
     1681 GTATGGGTTG TACCATGTCA CAGGAGGAGC GTGCCGCTCT TGAAAGATCA CGAATGTCCT
     1741 CAGTCTCCAA Ggtaacactt agtttttgtt gctctgttta aaaattaatt ttaagGGAGA
     1801 GGAGCTCATC AAGGAGAACA TGCACATGAA GCTCTACATG GAGGGAACCG TCAACAACCA
     1861 CCACTTCAAG TGCACCTCCG AGGGAGAGGG AAAGCCATAC GAGGGAACCC AAACCATGCG
     1921 TATCAAGGTC GTCGAGGGAG GACCACTCCC ATTCGCCTTC GACATCCTCG CCACCTCCTT
     1981 CATGTACGGA TCGCGTACCT TCATCAACCA CACCCAAGgt aagttttctt atgggaaaga
     2041 aggaaaaaac cgagatttta cttgaaaaat tgaatttttc gcgggatttt caccaaaaat
     2101 tgttgaatat tcattatttc acgctgtaaa acaaaaaaaa aaaaaatcaa aaactacgtt
     2161 gaaatcgcgt ttttaagcga attttcttca gaattgccag attttaaccc caaattttgc
     2221 agtttttaaa taaaatttca ccttttcggc tcaaattgta gattttcctg aaaatttagt
     2281 acaaaaaaca atttcctcgt aaatttttca aatagatttt cagGAATCCC AGACTTCTTC
     2341 AAGCAATCCT TCCCAGAGGG ATTCACCTGG GAGCGTGTCA CCACCTACGA GGACGGAGGA
     2401 GTCCTCACCG CCACCCAAGA CACCTCCCTC CAAGACGGAT GCCTCATCTA CAACGTCAAG
     2461 gtaagttatg tctacctgcc tgcctaccgc ctaaattttt tgtgaagttt cttcaaaaaa
     2521 tccagaaaaa aaaacaattt tcatacgatt ttttccctta aaattgtgaa ttttcatgct
     2581 ttttagcccc aaaagtcatt atttgagaaa aaatttcata caaaaaagtt ttgagaaata
     2641 cacaattttt taaatgtaat tttcaaattt tcaattttca actagaaaat tcacaaaact
     2701 tgtaaatttt ggaccaaaac atttatacaa ttactttttt tgaatctaat aactacaata
     2761 actacaaatt taacattttc agATCCGTGG AGTCAACTTC CCATCCAACG GACCAGTCAT
     2821 GCAAAAGAAG ACCCTCGGAT GGGAGGCCAA CACCGAGATG CTCTACCCAG CCGACGGAGG
     2881 ACTGGAGGGA CGTACCGACA TGGCCCTCAA GCTCGTCGGA GGAGGACACC TCATCTGCAA
     2941 CTTCAAGACC ACCTACCGTT CCAAGAAGCC AGCCAAGgta agttcttttt ttgaaaagtg
     3001 cagtttgtag tctaattttc atttattttc tttttaaaaa acgcatcaat ttttaaatat
     3061 tttgggacaa aatccgaaaa ctgtactaat ttgtagtttg taaaattaaa aaaaaaacgc
     3121 aaaaaaatgt tttcaaaaat gcttagaata aaaataaaat ttctaaaaaa ttgaacaaaa
     3181 taaacattta aaaattcaaa aagtttgaaa aaatgcaaag ttttttaatg caaaaaatat
     3241 ttaatgttta aacaaattta aaaaatgttg taagaatatg tttagagaca gtttttaaat
     3301 gttaaattgc aaaaaaaaaa agcaaaaaaa aatttgaaaa atgtaacaga aaattttaat
     3361 taaaaaagtc gtgttttttt caaacctgca attctttgta attttattta taaagagaaa
     3421 caaatcaaat tgtatattac gaaaaacgat actaaaattc gaaaatttgc ggttttttgc
     3481 gtaaaaaata cggttccgta attttcagAA CCTCAAGATG CCAGGAGTCT ACTACGTCGA
     3541 CCACCGTCTG GAGCGTATCA AGGAGGCCGA CAAGGAGACG TACGTCGAGC AACACGAGGT
     3601 CGCCGTCGCC CGTTACTGCG ACCTCCCATC CAAGCTCGGA CACAAGCTCA ACGGAATGGA
     3661 CGAGCTCTAC AAGACGcGAA GAGCAAGCTT GGATCGACGA GAGCAGCGCG ACTGGATCTG
     3721 TCGCCCGTCT CAAACGCAAC CCTCCGGCGG TCGCATATCA TTCAGGACGA GCCTCAGACT
     3781 CCAGCGTAAC TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG ATCAGCTAGA
     3841 ATCAAGCTGA CTAGATAAAC TGGCCGTCGT TTTACACGGG  
//
