LOCUS       _DR274_act_4_3_U        2479 bp    DNA        circular     04-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     DR274 act-4 3'UTR AAG GTA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    8..429
                     /locus_tag="act-4 3'UTR"
                     /label="act-4 3'UTR"
                     /ApEinfo_label="act-4 3'UTR"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(780..797)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(837..1424)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2..4
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1487..2302)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    599..605
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2381..2400
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gAAGTAAatt ttttgcccct tccacccaca acgctcaaaa gaagttcgta ttatcccgca
       61 acccatccag accatccttt tacatttttt ttattttctc tttttttttt cttcaccctc
      121 tccgtgtaca ttgctagtaa tagttccaac ttctctagcc gactgcatta tgcttcccat
      181 tctcacccaa atcttcattg gtaacttctc ttgtatttgt tcaatactat tccggtagta
      241 atctaggcat gtataactag gttctctgtg tatctttggt ttgaagttga ggtttaatgc
      301 aatcactttt gttgttgtac cctattactg ccgatgaccc ccattttctc attacaaaat
      361 tgacatttta aaccattttt tctctgaatc atgctcatta tttgagccac attcataata
      421 aagcttttaa taagacaaat tgatcaagtt tatctaatgt aaaaaataca aataaagaaa
      481 attcaacccg gaggagagat gaagtgagta gctgttacgg aacacagatt atttgccttg
      541 tttgtttccg aagatgtctc cgacgcggtc caaaattgag ccaatactga gcggGTAgGA
      601 AGAGCAAGCT TGGATCGACG AGAGCAGCGC GACTGGATCT GTCGCCCGTC TCAAACGCAA
      661 CCCTCCGGCG GTCGCATATC ATTCAGGACG AGCCTCAGAC TCCAGCGTAA CTGGACTGCA
      721 ATCAACTCAC TGGCTCACCT TCCGGTCCAC GATCAGCTAG AATCAAGCTG ACTAGATAAA
      781 CTGGCCGTCG TTTTACACGG GTGGGCCTTT CTTCGGTAGA AAATCAAAGG ATCTTCttga
      841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      901 gtggtttgtt tgccggatca agagctacca actctttttc cgaggtaact ggcttcagca
      961 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1381 atcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaAACGCC AGCAACGCAG
     1441 AAAGGCCCAC CCGAAGGTGA GCCAGGTGAT TACATTTAGG TCCTCATTAG AAAAACTCAT
     1501 CGAGCATCAA GTGAAACTGC AATTTATTCA TATCAGGATT ATCAATACCA TATTTTTGAA
     1561 AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA GTTCCATAGG ATGGCAAGAT
     1621 CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT ACAACCTATT AATTTCCCCT
     1681 CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT GACGACTGAA TCCGGTGAGA
     1741 ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC AGGCCAGCCA TTACGCTCGT
     1801 CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG TGATTGCGCC TGAGCGAGAC
     1861 GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG AATCGAATGC AACCGGCGCA
     1921 GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC AGGATATTCT TCTAATACCT
     1981 GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA TGCATCATCA GGAGTACGGA
     2041 TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG CCAGTTTAGC CTGACCATCT
     2101 CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT CAGAAACAAC TCTGGCGCAT
     2161 CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG CCCGACATTA TCGCGAGCCC
     2221 ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA TCGCGGCTTC AAGCAAGACG
     2281 TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GATAACTCAA
     2341 AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA gttggaacct cttacgtgcc
     2401 GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC TGCTATGGAG GTCAGGTATG
     2461 ATTTttGAAT TCGCTCTTC    
//
