LOCUS       DR274_tTAiS_L_QF        3098 bp    DNA        circular     21-SEP-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(686..1501)
                     /locus_tag="KanR2"
                     /label="KanR2"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3077..3094)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2265..2267
                     /locus_tag="rtTA-Q"
                     /label="rtTA-Q"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2619..2891
                     /locus_tag="sequenced 6735"
                     /label="sequenced 6735"
                     /ApEinfo_label="sequenced 6735"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    2171..2264
                     /locus_tag="rtTA-Q(1)"
                     /label="rtTA-Q(1)"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2896..2902
                     /locus_tag="< SapI "
                     /label="< SapI "
                     /ApEinfo_label="< SapI "
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2354
                     /locus_tag="rtTA-Q(2)"
                     /label="rtTA-Q(2)"
                     /ApEinfo_label="rtTA-Q"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2873..2891)
                     /locus_tag="NMoligo 6724"
                     /label="NMoligo 6724"
                     /ApEinfo_label="NMoligo 6724"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2618
                     /locus_tag="sequenced 4618"
                     /label="sequenced 4618"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     primer_bind     complement(2870..2888)
                     /locus_tag="NMoligo 6734"
                     /label="NMoligo 6734"
                     /ApEinfo_label="NMoligo 6734"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(36..623)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2265..2267
                     /locus_tag="rTetR"
                     /label="rTetR"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2386..2404
                     /locus_tag="NMoligo 6723"
                     /label="NMoligo 6723"
                     /ApEinfo_label="NMoligo 6723"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2171..2264
                     /locus_tag="rTetR(1)"
                     /label="rTetR(1)"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2388..2891
                     /locus_tag="QF activation domain"
                     /label="QF activation domain"
                     /ApEinfo_label="QF activation domain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1580..1599
                     /locus_tag="NMoligo 5008"
                     /label="NMoligo 5008"
                     /ApEinfo_label="NMoligo 5008"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2265..2267
                     /locus_tag="sequenced 4618(1)"
                     /label="sequenced 4618(1)"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    2171..2264
                     /locus_tag="sequenced 4618(2)"
                     /label="sequenced 4618(2)"
                     /ApEinfo_label="sequenced 4618"
                     /ApEinfo_fwdcolor="#5e5e5e"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    2268..2354
                     /locus_tag="rTetR(2)"
                     /label="rTetR(2)"
                     /ApEinfo_label="rTetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1672..1678
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2265..2267
                     /locus_tag="R TO T VARIENT arg in rtTA his in tTA "
                     /label="R TO T VARIENT arg in rtTA his in tTA "
                     /ApEinfo_label="R TO T VARIENT arg in rtTA his in tTA "
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1680..2170
                     /locus_tag="tTA"
                     /label="tTA"
                     /ApEinfo_label="tTA"
                     /ApEinfo_fwdcolor="#0096ff"
                     /ApEinfo_revcolor="#0096ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2358..2387
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#a9a9a9"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2873..2891)
                     /locus_tag="NMoligo 6711"
                     /label="NMoligo 6711"
                     /ApEinfo_label="NMoligo 6711"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2241..2243
                     /locus_tag="R TO T VARIENT lys in rtTA Arg in tTA"
                     /label="R TO T VARIENT lys in rtTA Arg in tTA"
                     /ApEinfo_label="R TO T VARIENT lys in rtTA Arg in tTA"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2172..2174
                     /locus_tag="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /label="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /ApEinfo_label="T TO T VARIENT Glu in rtTA Asp in tTA"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2012..2012
                     /locus_tag="introduced stop in intron"
                     /label="introduced stop in intron"
                     /ApEinfo_label="introduced stop in intron"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2014..2014
                     /locus_tag="introduces stop in intron"
                     /label="introduces stop in intron"
                     /ApEinfo_label="introduces stop in intron"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1668..2636
                     /locus_tag="sequenced 5008"
                     /label="sequenced 5008"
                     /ApEinfo_label="sequenced 5008"
                     /ApEinfo_fwdcolor="#797979"
                     /ApEinfo_revcolor="#797979"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     misc_feature    2688..2907
                     /locus_tag="sequenced 6133"
                     /label="sequenced 6133"
                     /ApEinfo_label="sequenced 6133"
                     /ApEinfo_fwdcolor="#797979"
                     /ApEinfo_revcolor="#5e5e5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
ORIGIN
        1 TGGGCCTTTC TTCGGTAGAA AATCAAAGGA TCTTCttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG
      661 CCAGGTGATT ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA
      721 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
      781 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
      841 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
      901 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAGAGT TTATGCATTT
      961 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCACCAA
     1021 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCCGTTAA
     1081 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     1141 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCTGGGA
     1201 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     1261 GAGGCATAAA TTCCGTCAGC CAGTTTAGCC TGACCATCTC ATCTGTAACA TCATTGGCAA
     1321 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     1381 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     1441 CATCCATGTT GGAATTTAAT CGCGGCTTCA AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     1501 TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC
     1561 TTATTTCATT ATGGTGAAAg ttggaacctc ttacgtgccG ATCAAGTCAA AAGCCTCCGG
     1621 TCGGAGGCTT TTGACTTTCT GCTATGGAGG TCAGGTATGA TTTttGAATT CGCTCTTCgA
     1681 TGTCACGTCT AGATAAATCC AAGGTCATCA ACTCCGCCCT CGAGCTCCTC AATGAGGTCG
     1741 GAATTGAGGG ACTCACCACC CGTAAGCTCG CCCAAAAGCT CGGAGTTGAG CAACCAACCC
     1801 TCTACTGGCA CGTCAAGAAC AAGCGTGCAC TTCTCGACGC CCTCGCCATC GAGATGCTCG
     1861 ATCGTCACCA CACCCACTTC TGCCCACTCG AGGGAGAATC CTGGCAAGAT TTCCTCCGTA
     1921 ACAACGCCAA GTCCTTCCGT TGCGCCCTTC TCTCCCACCG TGACGGAGCC AAGgtaagtt
     1981 taaacatata tatactaact aaccctgatt aatgaaattt tcagGTCCAT CTCGGAACCC
     2041 GTCCAACCGA GAAGCAATAC GAGACTCTCG AGAACCAACT TGCCTTCTTG TGCCAACAAG
     2101 GATTCTCCCT CGAAAACGCC CTCTACGCCC TCTCCGCCGT CGGACATTTC ACCCTCGGAT
     2161 GCGTCCTCGA AGATCAAGAA CATCAAGTTG CAAAAGAAGA ACGAGAAACT CCAACAACTG
     2221 ATTCAATGCC ACCACTTTTG AGACAAGCTA TCGAACTTTT CGATCACCAA GGAGCAGAAC
     2281 CAGCTTTCCT TTTTGGACTG GAACTTATTA TCTGTGGACT TGAGAAACAA CTGAAATGTG
     2341 AATCGGGAGG AGGAGCGGGT TCTGGATCTG GATCTGGAGG TTCCGGTCGT CAACTTGAGC
     2401 TCCCACCAAC CGCCACCGCC ACCGCCAGTA TCATGCCACA CGTCATGGAG CAACCACTCT
     2461 CCACCTCCAT CAACCCAGTC AACGACCGTT TCAACGGAAT ACCAAACCCA ACCCCATACA
     2521 ACTCCGACGC CGCCCTCGAC GCCATCACCC AAACCAACGA CTACGGATCC GTCAACACCC
     2581 ACGGAATCCT CTCCACCTAC CCACCACCAG CCACCCACCT CAACGAGGCC TCCGTCGCCC
     2641 TCGCACCAGG AGGAGCACCA CCACGTCCAC CACCACCATA CGTCGACTCC ACCACCAACC
     2701 ACCCACCATA CCATTCCAAC CTCGTCCCAA TGGCCAACTT CGGATACTCC ACCGTCGACT
     2761 ACGACGCCAT GGTCGACGAC CTCGCCTCCA TCGAGTACAC CGACGCCGTC GACGTCGACC
     2821 CACAATTCAT GACCAACCTC GGGTTCGTCC CAGGATGCAA CTTCTCCGAC ATCAACACCT
     2881 ATGAGCAATA AAAGgGAAGA GCAAGCTTGG ATCGACGAGA GCAGCGCGAC TGGATCTGTC
     2941 GCCCGTCTCA AACGCAACCC TCCGGCGGTC GCATATCATT CAGGACGAGC CTCAGACTCC
     3001 AGCGTAACTG GACTGCAATC AACTCACTGG CTCACCTTCC GGTCCACGAT CAGCTAGAAT
     3061 CAAGCTGACT AGATAAACTG GCCGTCGTTT TACACGGG  
//
