LOCUS       pHygG12__4613_          6032 bp    DNA        circular     22-SEP-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1008..1667)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3671..4072
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2660..2662
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2799..2849
                     /locus_tag="Synthetic Intron 1"
                     /label="Synthetic Intron 1"
                     /ApEinfo_label="Synthetic Intron 1"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2979..3029
                     /locus_tag="Synthetic Intron 2"
                     /label="Synthetic Intron 2"
                     /ApEinfo_label="Synthetic Intron 2"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(2693..2798,2850..2978,3030..3160,3212..3436,
                     3488..3607)
                     /locus_tag="NeonGreen_Cel"
                     /label="NeonGreen_Cel"
                     /ApEinfo_label="NeonGreen_Cel"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 3608..3664
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3437..3487
                     /locus_tag="Synthetic Intron 4"
                     /label="Synthetic Intron 4"
                     /ApEinfo_label="Synthetic Intron 4"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4199..4550)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     2660..2692
                     /locus_tag="SV40 nls"
                     /label="SV40 nls"
                     /ApEinfo_label="SV40 nls"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(5644..6006)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3161..3211
                     /locus_tag="Synthetic Intron 3"
                     /label="Synthetic Intron 3"
                     /ApEinfo_label="Synthetic Intron 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2569..2602)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4576..5601)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2535..2569
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2128..2434)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     2623..2656
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     4552..4572
                     /locus_tag="attB2"
                     /label="attB2"
                     /ApEinfo_label="attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     complement(5607..5627)
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4559..4565
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(5614..5620)
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2463..2496
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2463..2496
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(223..910)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
       61 gcgaattccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      481 cgctccaagc tgggctgtgt gcacgaaccc cccattcagc ccgaccgctg cgccttatcc
      541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1141 ccgcgagatc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1981 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2101 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2161 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2221 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2281 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2341 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2401 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcgtcggagc
     2461 tcATAACTTC GTATAGCATA CATTATACGA AGTTATgcgg ccgctctaga aCTAGTGGAA
     2521 CCGAAGAGCC TGCAggtaac cACGCGttGC atGCTCTTCG GTAcccggGA AGTTCCTATA
     2581 CtatttgaaG AATAGGAACT TCtgcaggaa ttcgatatcG GTGAAGTTCC TATTCtctag
     2641 aaaGTATAGG AACTTCAAAA TGGGATCCCC AAAGAAGAAG CGTAAGGTGT CGATGGTGTC
     2701 GAAGGGAGAA GAGGATAACA TGGCTTCACT CCCAGCTACA CACGAACTCC ACATCTTCGG
     2761 ATCGATCAAC GGAGTGGATT TCGATATGGT CGGACAAGgt aagtttaaac atatatatac
     2821 taactaaccc tgattattta aattttcagG AACTGGAAAC CCAAACGATG GATACGAGGA
     2881 ACTCAACCTC AAGTCGACAA AGGGAGATCT GCAATTCTCG CCATGGATTC TCGTGCCACA
     2941 CATCGGATAC GGATTCCACC AATACCTCCC ATACCCAGgt aagtttaaac tgagttctac
     3001 taactaacga gtaatattta aattttcagA TGGAATGTCA CCATTCCAAG CTGCCATGGT
     3061 GGATGGATCG GGATACCAAG TTCACCGAAC AATGCAATTC GAGGATGGAG CCTCGCTCAC
     3121 AGTGAACTAC CGATACACAT ACGAGGGATC GCACATCAAG gtaagtttaa acagttcggt
     3181 actaactaac catacatatt taaattttca gGGAGAGGCT CAAGTTAAGG GAACAGGATT
     3241 CCCAGCTGAT GGACCAGTGA TGACAAACTC ACTCACAGCT GCTGATTGGT GCCGATCGAA
     3301 AAAGACATAC CCAAATGATA AGACAATCAT CTCGACATTC AAGTGGTCGT ACACTACTGG
     3361 AAACGGAAAG CGATACCGAT CGACAGCCCG AACAACATAC ACATTCGCTA AGCCAATGGC
     3421 CGCCAACTAC CTCAAGgtaa gtttaaacat gattttacta actaactaat ctgatttaaa
     3481 ttttcagAAT CAACCAATGT ACGTGTTCCG AAAGACAGAA CTCAAGCACT CAAAGACAGA
     3541 GCTGAACTTC AAAGAGTGGC AAAAGGCCTT CACAGATGTG ATGGGAATGG ATGAACTCTA
     3601 CAAGGCCTCC CGTCGTCGTA AGGCCAACCC AACCAAGCTC TCCGAGAACG CCAAGAAGCT
     3661 CGCCAAGtag atcaactgct ctaattccat ctcgtttctc tcttttcttt caatctcttt
     3721 acccgtttct cttcccctac gtcccccaaa cctaccactg cacagtaaca catacacatt
     3781 caacgtgtca caataaaagc ccatcaacac cttctctgtt gttttttttc ttcaaatgca
     3841 tttttttcac tttgtttaat aatttttacg catcggattt gtaggatagt ggaaatttgt
     3901 ccttcaatgc ttcaattgat tcgacacttc tcaatatttt tgatcaataa accattgatt
     3961 ttctatttag aagaagatca ctgtaaataa caccaaaaac atcacagcaa aaaaactccc
     4021 actcaattat attactgttt ttattgaaac tagaataaat ttgaaaatct tcaaagcata
     4081 cacaattaaa aaaaacgaaa aaagaaaaag tcccgttgat tctcaaactt aaatttttaa
     4141 acttgtctga accatgttgt aagtttgtaa aagtttccca gttgtcctcg aacaGGTCTA
     4201 AAAATTGCGG TCATAAACTG AAACGTAACA TATGATAAGG TATTTTGTGT GCGGTTTTTT
     4261 CTATGATGTT TCAAAAAAAT TTATCAGAAG TAAAAAACAT AAGAAGTGTA CACAGAACAT
     4321 TGTGTAGATT TTGGGATAGA ATTATTATTA ATGGGGGGAG GGAGCACAAT TTTTTTCGAC
     4381 TTTTTATTAC GAATTAATTC TATTTTTGAA TCTACACAAT TTCATTGTTA GAGGTGACTT
     4441 AAAAGAAGCT AAAAAACAAA GAAATTAAGA GAAGTTTTTT GATAATAACA AAAATAGGGG
     4501 GTGGGAGCAC AGGGAGAAAG AGCATGTAGG GATGTTGAAG AGTAATTGGA CCCACTTTGT
     4561 ACAAGAAAGC TGGGTCTATT CCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT
     4621 ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG
     4681 TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA
     4741 AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG
     4801 GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA
     4861 GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC
     4921 GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT
     4981 GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA
     5041 GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC
     5101 GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG
     5161 CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT
     5221 AAGATCGGCC GCAGCGATCG CATCCATGGC CTCCGCGACC GGCTGCAGAA CAGCGGGCAG
     5281 TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT
     5341 CAGGCTCTCG CTGAATTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC
     5401 AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG
     5461 GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG
     5521 CTGCATCAGG TCGGAGACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC
     5581 GGTGAGTTCA GGCTTTTTCA TGGAGCCTGC TTTTTTGTAC AAACTTGCGG CACCGCCTGA
     5641 CATATTACCT TAAAATTCAA AAATTAATTT CAGATCAGTC ATAACCAAGA AAACAAAATA
     5701 AACGTTAGGA CAGAAAATTC AAATTAATTA AAAATTATGC TGAATAAGAA AATTACATGC
     5761 AAAGCCTTAT TTTCCTCATA GAAACAGTTG AATAACATAT TTTTTAATTG AAAACATAGC
     5821 AAAATTCGTG GTAAAATTTG TCAAAACCTT GGAAAAAATC GAAAAAAGAA CATTTCCCGA
     5881 TGCATTTTCC TATTTCTCGT TGCCGACAAC GTATAAACGT TGAATACTCA GTCAACGAAT
     5941 TTGGCTGCTG GCTTGGACTC TCGCACGGAA GAGACGCGTG TGTAGATTTA CGACGAAAGC
     6001 AAAAATgatc ccgctttcca gtcgggaaac ct  
//
