LOCUS       pHygR12__4615_          5969 bp    DNA        circular     22-SEP-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1033..1692)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3876..4034
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3633..3875
                     /locus_tag="myo-2 3' UTR(1)"
                     /label="myo-2 3' UTR(1)"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4161..4512)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5606..5968)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  2688..2693
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(4538..5563)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(2718..2870,2922..3134,3186..3356,3408..3563)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2718..2720
                     /locus_tag="ATG Overlap"
                     /label="ATG Overlap"
                     /ApEinfo_label="ATG Overlap"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2153..2459)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 3570..3626
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     4514..4534
                     /locus_tag="attB2"
                     /label="attB2"
                     /ApEinfo_label="attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     complement(5569..5589)
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4521..4527
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(5576..5582)
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2488..2521
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2488..2521
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2648..2681
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2685..2687
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2594..2627)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2560..2594
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     2694..2716
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(248..935)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 atcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
       61 cggggagagg cggtttgcgt attgggcgaa ttccgcttcc tcgctcactg actcgctgcg
      121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccat
      541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1141 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc
     1201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     2041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2461 ccgctacagg gcgcgtcgtc ggagctcATA ACTTCGTATA GCATACATTA TACGAAGTTA
     2521 Tgcggccgct ctagaaCTAG TGGAACCGAA GAGCCTGCAg gtaaccACGC GttGCatGCT
     2581 CTTCGGTAcc cggGAAGTTC CTATACtatt tgaaGAATAG GAACTTCtgc aggaattcga
     2641 tatcGGTGAA GTTCCTATTC tctagaaaGT ATAGGAACTT CAAAATGGGA TCCCCAAAGA
     2701 AGAAGCGTAA GGTGTCgATG GTTTCCAAGG GAGAGGCCGT CATCAAGGAG TTCATGCGTT
     2761 TCAAGGTCCA CATGGAGGGA TCCATGAACG GACACGAGTT CGAGATCGAG GGAGAGGGAG
     2821 AGGGACGTCC ATACGAGGGA ACCCAAACCG CCAAGCTCAA GGTCACCAAG gtaagtttaa
     2881 acatatatat actaactaac cctgattatt taaattttca gGGAGGACCA CTCCCATTCT
     2941 CCTGGGACAT CCTCTCCCCA CAATTCATGT ACGGATCCCG TGCCTTCACC AAGCACCCAG
     3001 CCGACATCCC AGACTACTAC AAGCAATCCT TCCCAGAGGG ATTCAAGTGG GAGCGTGTCA
     3061 TGAACTTCGA GGACGGAGGA GCCGTCACCG TCACCCAAGA CACCTCCCTC GAGGACGGAA
     3121 CCCTCATCTA CAAGgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     3181 ttcagGTCAA GCTCCGTGGA ACCAACTTCC CACCAGACGG ACCAGTCATG CAAAAGAAGA
     3241 CCATGGGATG GGAGGCCTCC ACCGAGCGTC TCTACCCAGA GGACGGAGTC CTCAAGGGAG
     3301 ACATCAAGAT GGCCCTCCGT CTCAAGGACG GAGGACGTTA CCTCGCCGAC TTCAAGgtaa
     3361 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagACC ACCTACAAGG
     3421 CCAAGAAGCC AGTCCAAATG CCAGGAGCCT ACAACGTCGA CCGTAAGCTC GACATCACCT
     3481 CCCACAACGA GGACTACACC GTCGTCGAGC AATACGAGCG TTCCGAGGGA CGTCACTCCA
     3541 CCGGAGGAAT GGACGAGCTC TACAAGGCCT CCCGTCGTCG TAAGGCCAAC CCAACCAAGC
     3601 TCTCCGAGAA CGCCAAGAAG CTCGCCAAGt agatcaactg ctctaattcc atctcgtttc
     3661 tctcttttct ttcaatctct ttacccgttt ctcttcccct acgtccccca aacctaccac
     3721 tgcacagtaa cacatacaca ttcaacgtgt cacaataaaa gcccatcaac accttctctg
     3781 ttgttttttt tcttcaaatg catttttttc actttgttta ataattttta cgcatcggat
     3841 ttgtaggata gtggaaattt gtccttcaat gcttcaattg attcgacact tctcaatatt
     3901 tttgatcaat aaaccattga ttttctattt agaagaagat cactgtaaat aacaccaaaa
     3961 acatcacagc aaaaaaactc ccactcaatt atattactgt ttttattgaa actagaataa
     4021 atttgaaaat cttcaaagca tacacaatta aaaaaaacga aaaaagaaaa agtcccgttg
     4081 attctcaaac ttaaattttt aaacttgtct gaaccatgtt gtaagtttgt aaaagtttcc
     4141 cagttgtcct cgaacaGGTC TAAAAATTGC GGTCATAAAC TGAAACGTAA CATATGATAA
     4201 GGTATTTTGT GTGCGGTTTT TTCTATGATG TTTCAAAAAA ATTTATCAGA AGTAAAAAAC
     4261 ATAAGAAGTG TACACAGAAC ATTGTGTAGA TTTTGGGATA GAATTATTAT TAATGGGGGG
     4321 AGGGAGCACA ATTTTTTTCG ACTTTTTATT ACGAATTAAT TCTATTTTTG AATCTACACA
     4381 ATTTCATTGT TAGAGGTGAC TTAAAAGAAG CTAAAAAACA AAGAAATTAA GAGAAGTTTT
     4441 TTGATAATAA CAAAAATAGG GGGTGGGAGC ACAGGGAGAA AGAGCATGTA GGGATGTTGA
     4501 AGAGTAATTG GACCCACTTT GTACAAGAAA GCTGGGTCTA TTCCTTTGCC CTCGGACGAG
     4561 TGCTGGGGCG TCGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG GTCCAGACGG
     4621 CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCGGAT CGGACGATTG
     4681 CGTCGCATCG ACCCTGCGCC CAAGCTGCAT CATCGAAATT GCCGTCAACC AAGCTCTGAT
     4741 AGAGTTGGTC AAGACCAATG CGGAGCATAT ACGCCCGGAG CCGCGGCGAT CCTGCAAGCT
     4801 CCGGATGCCT CCGCTCGAAG TAGCGCGTCT GCTGCTCCAT ACAAGCCAAC CACGGCCTCC
     4861 AGAAGAAGAT GTTGGCGACC TCGTATTGGG AATCCCCGAA CATCGCCTCG CTCCAGTCAA
     4921 TGACCGCTGT TATGCGGCCA TTGTCCGTCA GGACATTGTT GGAGCCGAAA TCCGCGTGCA
     4981 CGAGGTGCCG GACTTCGGGG CAGTCCTCGG CCCAAAGCAT CAGCTCATCG AGAGCCTGCG
     5041 CGACGGACGC ACTGACGGTG TCGTCCATCA CAGTTTGCCA GTGATACACA TGGGGATCAG
     5101 CAATCGCGCA TATGAAATCA CGCCATGTAG TGTATTGACC GATTCCTTGC GGTCCGAATG
     5161 GGCCGAACCC GCTCGTCTGG CTAAGATCGG CCGCAGCGAT CGCATCCATG GCCTCCGCGA
     5221 CCGGCTGCAG AACAGCGGGC AGTTCGGTTT CAGGCAGGTC TTGCAACGTG ACACCCTGTG
     5281 CACGGCGGGA GATGCAATAG GTCAGGCTCT CGCTGAATTC CCCAATGTCA AGCACTTCCG
     5341 GAATCGGGAG CGCGGCCGAT GCAAAGTGCC GATAAACATA ACGATCTTTG TAGAAACCAT
     5401 CGGCGCAGCT ATTTACCCGC AGGACATATC CACGCCCTCC TACATCGAAG CTGAAAGCAC
     5461 GAGATTCTTC GCCCTCCGAG AGCTGCATCA GGTCGGAGAC GCTGTCGAAC TTTTCGATCA
     5521 GAAACTTCTC GACAGACGTC GCGGTGAGTT CAGGCTTTTT CATGGAGCCT GCTTTTTTGT
     5581 ACAAACTTGC GGCACCGCCT GACATATTAC CTTAAAATTC AAAAATTAAT TTCAGATCAG
     5641 TCATAACCAA GAAAACAAAA TAAACGTTAG GACAGAAAAT TCAAATTAAT TAAAAATTAT
     5701 GCTGAATAAG AAAATTACAT GCAAAGCCTT ATTTTCCTCA TAGAAACAGT TGAATAACAT
     5761 ATTTTTTAAT TGAAAACATA GCAAAATTCG TGGTAAAATT TGTCAAAACC TTGGAAAAAA
     5821 TCGAAAAAAG AACATTTCCC GATGCATTTT CCTATTTCTC GTTGCCGACA ACGTATAAAC
     5881 GTTGAATACT CAGTCAACGA ATTTGGCTGC TGGCTTGGAC TCTCGCACGG AAGAGACGCG
     5941 TGTGTAGATT TACGACGAAA GCAAAAATg   
//
