LOCUS       pU119F1                 4814 bp    DNA        circular     22-SEP-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     ATCC vector GGTC PCR from 8 (BsaI) to 2699 (BsaI) 
COMMENT     GGTC unc-119 ATCC PCR from 8 (BsaI) to 2129 (BsaI) 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(3203..3862)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     38..48
                     /locus_tag="SV40 nls"
                     /label="SV40 nls"
                     /ApEinfo_label="SV40 nls"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(4761..4794)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4707..4761
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2418..3105)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(4323..4629)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     1..34
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4658..4691
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     4658..4691
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2171..2189
                     /note=") PCR_conditions {primer
                     sequence:TGGTCTCaatcccgctttccagtcggg}"
                     /locus_tag="7192"
                     /label="7192"
                     /ApEinfo_label="7192"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    38..40
                     /locus_tag="pMYO-2"
                     /label="pMYO-2"
                     /ApEinfo_label="pMYO-2"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            53..2166
                     /locus_tag="C.Briggsae unc-119(+)"
                     /label="C.Briggsae unc-119(+)"
                     /ApEinfo_label="C.Briggsae unc-119(+)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1041..1886
                     /locus_tag="CB-unc-119 CDS"
                     /label="CB-unc-119 CDS"
                     /ApEinfo_label="CB-unc-119 CDS"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            2167..2170
                     /locus_tag="C.Briggsae unc-119(+)(1)"
                     /label="C.Briggsae unc-119(+)(1)"
                     /ApEinfo_label="C.Briggsae unc-119(+)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     49..52
                     /PCR_conditions="primer
                     sequence:tGGTCTCaGGTCcATTTTTGCTTTCGTCGTAAATC"
                     /locus_tag="newHygF"
                     /label="newHygF"
                     /ApEinfo_label="newHygF"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GAAGTTCCTA TTCtctagaa aGTATAGGAA CTTCAAAATG GGATCCCCGG TCctttgagc
       61 caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa aatgtttatt
      121 ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca gaaaccggga
      181 tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac aaaccggtta
      241 ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca aattaagtta
      301 catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta ttctgttcct
      361 attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc ttggatagtg
      421 tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt ccgctcgttc
      481 ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct tctcaatctt
      541 cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac cctcattcat
      601 ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc tattccacgt
      661 cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat tcgtttttgg
      721 gattcacccc gcggttcgca acaggtttct ttttcaaatt attagcgtta taaatagaaa
      781 aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta catgtactca
      841 aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca ttatcgtatt
      901 tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt ggcaccctct
      961 aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt ccgttttcag
     1021 ccgcttccaa ccaaaccgat ATGAAAGCCG AGCAACAACA ATCGATTCCA CCCGGCTCGG
     1081 CGACCTTCCC GTCGCAGGTG AGACTCAGAA AACTAGAGAA ACCGCTCAAC TAACTCTTGA
     1141 TATCCGATTT CATTCTTTTC TCTTTTCTTT TTGTTGAACT TTTCCATATT TCCAGATGCC
     1201 ACGGCCACCA CCAAGCACCG AACAAGGAAT CACAACGGAA TCGGAGCTTG CGAAGAAAGC
     1261 TCAAATCACT CCGAACGACG TTTTAGCACT TCCGGGAATC ACTCAAGGTA TCTTGCTCCC
     1321 TTTGGATTTC AGTTTAAAAC ATAAATTTAG GATTCTTATG CTCCCCATCT GCGAACATCT
     1381 ATAACATCGA GTTCACCAAG TTCCAAATCC GTGATCTGGA CACTGAGCAA GTGCTTTTCG
     1441 AGATCGCCAA ACCGGAGAAC GATCAGGAGA ATGATGAGTC GCCACAGGAG TCGGCAAGAT
     1501 ACGTGCGTTA TAGATTTGCT CCAAACTTTT TGAAACTCAA AACGGTCGGA GCAACTGTGG
     1561 AATTCAAAGT AGGAGACATC CCAATCCATC ATTTCCGAAT GATCGAACGT CACTTCTTCA
     1621 AAGATCGCCT TCTGAAGTGT TTTGACTTTG AATTCGGATT CTGTATTCCG AATTCACGAA
     1681 ACAACTGTGA ACATATCTAT GAGTTCCCTC AACTCTCTCA ACAACTCAGT GAGTCATTAT
     1741 TCTAAAAAGT ACAATTCAAA AGACTAATCT CTTTTTCAGT GGACGACATG ATCAACAATC
     1801 CAAACGAGAC TCGTTCTGAC AGCTTCTATT TCGTCGATAA CAAACTCGTC ATGCACAACA
     1861 AAGCCGACTA CTCATATGAT GCATAAatat ttaatacaaa aatgttctgg ataattattc
     1921 tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg tctagtttgt
     1981 tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt ccccccttgt
     2041 ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca cggaaatctg
     2101 ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga ttttttcatt
     2161 agagaatgtc atcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2221 ggccaacgcg cggggagagg cggtttgcgt attgggcgaa ttccgcttcc tcgctcactg
     2281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc
     3361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3481 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3781 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     4201 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4261 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4321 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4381 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4441 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4501 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4561 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4621 gcttaatgcg ccgctacagg gcgcgtcgtc ggagctcATA ACTTCGTATA GCATACATTA
     4681 TACGAAGTTA Tgcggccgct ctagaaCTAG TGGCGAAGAG CCTGCAggta accACGCGtt
     4741 GCatGCTCTT CGGTAcccgg GAAGTTCCTA TACtatttga aGAATAGGAA CTTCtgcagg
     4801 aattcgatat cGGT    
//
