LOCUS       pHygRP2                 6547 bp    DNA        circular     01-DEC-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Ligation reaction:
COMMENT     pHygR2 [4388] from 3105 (XhoI*) to 2535 (SpeI)
COMMENT     pHygRP3 [4483] from 4367 (SpeI) to 5516 (XhoI*)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1033..1692)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2153..2459)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2488..2521
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2573..2793
                     /locus_tag="full attP "
                     /label="full attP "
                     /ApEinfo_label="full attP "
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4211..4612
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4739..5090)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(6147..6167)
                     /note="not related to phiC31"
                     /locus_tag="Gateway attB1"
                     /label="Gateway attB1"
                     /ApEinfo_label="Gateway attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(248..935)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     5092..5112
                     /note="not related to phiC31"
                     /locus_tag="Gateway attB2"
                     /label="Gateway attB2"
                     /ApEinfo_label="Gateway attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2687..2688
                     /locus_tag="recombination site"
                     /label="recombination site"
                     /ApEinfo_label="recombination site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(6184..6546)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3172..3205)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5116..6141)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3142..3166
                     /locus_tag="synthetic sGRNA site B"
                     /label="synthetic sGRNA site B"
                     /ApEinfo_label="synthetic sGRNA site B"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2799..3084
                     /locus_tag="rps-7 3' UTR"
                     /label="rps-7 3' UTR"
                     /ApEinfo_label="rps-7 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2666..2707
                     /locus_tag="attP phiC31"
                     /label="attP phiC31"
                     /ApEinfo_label="attP phiC31"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3226..3259
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 4148..4204
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5099..5105
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     sig_peptide     3272..3295
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(3296..3448,3500..3712,3764..3934,3986..4141)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6154..6160)
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2542..2564
                     /locus_tag="synthetic sgRNA site A"
                     /label="synthetic sgRNA site A"
                     /ApEinfo_label="synthetic sgRNA site A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3089..3141
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="#ff89d8"
                     /ApEinfo_revcolor="#ff89d8"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 atcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
       61 cggggagagg cggtttgcgt attgggcgaa ttccgcttcc tcgctcactg actcgctgcg
      121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccat
      541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1141 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc
     1201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     2041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2461 ccgctacagg gcgcgtcgtc ggagctcATA ACTTCGTATA GCATACATTA TACGAAGTTA
     2521 Tgcggccgct ctagaaCTAG TGGCTGACAT CCAGTAGACG GAGGctaCCG GTgtactgac
     2581 ggacacaccg aagccccggc ggcaaccctc agcggatgcc ccggggcttc acgttttccc
     2641 aggtcagaag cggttttcgg gagtaGTGCC CCAACTGGGG TAACCTTTGA GTTCTCTCAG
     2701 TTGGGGGcgt agggtcgccg acatgacaca aggggttgtg accggggtgg acacgtacgc
     2761 gggtgcttac gaccgtcagt cgcgcgagcg cgaCAATTGT CTAAACATTT TTTGTTGACT
     2821 TACAATAAAT TGTTGATATA ATTTATTGTG AAATGGGTAT TCTTGAATGA TTTGATTTTA
     2881 ACGAAGAGAA CGCTGAAATC AACTGAACAC CTCGAATTGA GATTTTGGGG ATTTTATACC
     2941 AAGTTATCGA CGACGCAAAT GGTGCAAACT TTATATTTTC AAATATTCTG GAAGCATTCA
     3001 ATGGTCTTTT AAGAAACGGA CAACTCCATT CTTGATATGC AATGTTTTTT AAAAGAAATC
     3061 TCTTTAATCG AGACGGGCGC ATATagaaCC TAGGTGGCGA AGAGCCTGCA ggtaaccACG
     3121 CGttGCatGC TCTTCCGTAC GGCGACGTAA CGTGAGATAG GCGGaacccg gGAAGTTCCT
     3181 ATACtatttg aaGAATAGGA ACTTCtgcag gaattcgata tcGGTGAAGT TCCTATTCtc
     3241 tagaaaGTAT AGGAACTTCa aaaTGGGATC CCCAAAGAAG AAGCGTAAGG TGTCGATGGT
     3301 TTCCAAGGGA GAGGCCGTCA TCAAGGAGTT CATGCGTTTC AAGGTCCACA TGGAGGGATC
     3361 CATGAACGGA CACGAGTTCG AGATCGAGGG AGAGGGAGAG GGACGTCCAT ACGAGGGAAC
     3421 CCAAACCGCC AAGCTCAAGG TCACCAAGgt aagtttaaac atatatatac taactaaccc
     3481 tgattattta aattttcagG GAGGACCACT CCCATTCTCC TGGGACATCC TCTCCCCACA
     3541 ATTCATGTAC GGATCCCGTG CCTTCACCAA GCACCCAGCC GACATCCCAG ACTACTACAA
     3601 GCAATCCTTC CCAGAGGGAT TCAAGTGGGA GCGTGTCATG AACTTCGAGG ACGGAGGAGC
     3661 CGTCACCGTC ACCCAAGACA CCTCCCTCGA GGACGGAACC CTCATCTACA AGgtaagttt
     3721 aaacagttcg gtactaacta accatacata tttaaatttt cagGTCAAGC TCCGTGGAAC
     3781 CAACTTCCCA CCAGACGGAC CAGTCATGCA AAAGAAGACC ATGGGATGGG AGGCCTCCAC
     3841 CGAGCGTCTC TACCCAGAGG ACGGAGTCCT CAAGGGAGAC ATCAAGATGG CCCTCCGTCT
     3901 CAAGGACGGA GGACGTTACC TCGCCGACTT CAAGgtaagt ttaaacatga ttttactaac
     3961 taactaatct gatttaaatt ttcagACCAC CTACAAGGCC AAGAAGCCAG TCCAAATGCC
     4021 AGGAGCCTAC AACGTCGACC GTAAGCTCGA CATCACCTCC CACAACGAGG ACTACACCGT
     4081 CGTCGAGCAA TACGAGCGTT CCGAGGGACG TCACTCCACC GGAGGAATGG ACGAGCTCTA
     4141 CAAGGCCTCC CGTCGTCGTA AGGCCAACCC AACCAAGCTC TCCGAGAACG CCAAGAAGCT
     4201 CGCCAAGtag atcaactgct ctaattccat ctcgtttctc tcttttcttt caatctcttt
     4261 acccgtttct cttcccctac gtcccccaaa cctaccactg cacagtaaca catacacatt
     4321 caacgtgtca caataaaagc ccatcaacac cttctctgtt gttttttttc ttcaaatgca
     4381 tttttttcac tttgtttaat aatttttacg catcggattt gtaggatagt ggaaatttgt
     4441 ccttcaatgc ttcaattgat tcgacacttc tcaatatttt tgatcaataa accattgatt
     4501 ttctatttag aagaagatca ctgtaaataa caccaaaaac atcacagcaa aaaaactccc
     4561 actcaattat attactgttt ttattgaaac tagaataaat ttgaaaatct tcaaagcata
     4621 cacaattaaa aaaaacgaaa aaagaaaaag tcccgttgat tctcaaactt aaatttttaa
     4681 acttgtctga accatgttgt aagtttgtaa aagtttccca gttgtcctcg aacaGGTCTA
     4741 AAAATTGCGG TCATAAACTG AAACGTAACA TATGATAAGG TATTTTGTGT GCGGTTTTTT
     4801 CTATGATGTT TCAAAAAAAT TTATCAGAAG TAAAAAACAT AAGAAGTGTA CACAGAACAT
     4861 TGTGTAGATT TTGGGATAGA ATTATTATTA ATGGGGGGAG GGAGCACAAT TTTTTTCGAC
     4921 TTTTTATTAC GAATTAATTC TATTTTTGAA TCTACACAAT TTCATTGTTA GAGGTGACTT
     4981 AAAAGAAGCT AAAAAACAAA GAAATTAAGA GAAGTTTTTT GATAATAACA AAAATAGGGG
     5041 GTGGGAGCAC AGGGAGAAAG AGCATGTAGG GATGTTGAAG AGTAATTGGA CCCACTTTGT
     5101 ACAAGAAAGC TGGGTCTATT CCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT
     5161 ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG
     5221 TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA
     5281 AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG
     5341 GAGCATATAC GCCCGGAGCC GCGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA
     5401 GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC
     5461 GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT
     5521 GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA
     5581 GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC
     5641 GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG
     5701 CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT
     5761 AAGATCGGCC GCAGCGATCG CATCCATGGC CTCCGCGACC GGCTGCAGAA CAGCGGGCAG
     5821 TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT
     5881 CAGGCTCTCG CTGAATTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC
     5941 AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG
     6001 GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG
     6061 CTGCATCAGG TCGGAGACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC
     6121 GGTGAGTTCA GGCTTTTTCA TGGAGCCTGC TTTTTTGTAC AAACTTGCGG CACCGCCTGA
     6181 CATATTACCT TAAAATTCAA AAATTAATTT CAGATCAGTC ATAACCAAGA AAACAAAATA
     6241 AACGTTAGGA CAGAAAATTC AAATTAATTA AAAATTATGC TGAATAAGAA AATTACATGC
     6301 AAAGCCTTAT TTTCCTCATA GAAACAGTTG AATAACATAT TTTTTAATTG AAAACATAGC
     6361 AAAATTCGTG GTAAAATTTG TCAAAACCTT GGAAAAAATC GAAAAAAGAA CATTTCCCGA
     6421 TGCATTTTCC TATTTCTCGT TGCCGACAAC GTATAAACGT TGAATACTCA GTCAACGAAT
     6481 TTGGCTGCTG GCTTGGACTC TCGCACGGAA GAGACGCGTG TGTAGATTTA CGACGAAAGC
     6541 AAAAATg     
//
